- 著者
- Shoichi YASUNO Takeomi MURATA Kazuko KOKUBO Takashi YAMAGUCHI Masugu KAMEI
- 出版者
- (社)日本農芸化学会
- 雑誌
- Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)
- 巻号頁・発行日
- vol.61, no.11, pp.1944-1946, 1997-11-23 (Released:2008-02-08)
- 参考文献数
- 8
- 被引用文献数
- 34 78
ρ-Aminobenzoic ethyl ester (ABEE)-derivatized monosaccharides were separated by HPLC with a trifluoroacetic acid (TFA) solution or borate buffer as the eluent. In the case of the TFA solution, ABEE-derivatized monosaccharides of the neutral and amino sugars found in animal glycoproteins were separated in a simultaneous analysis. In the case of the borate buffer, ABEE-derivatized monosaccharides of identical molecular weights such as ABEE-Gal, -Glc, and -Man were separated as stereoisomers. Glucuronic acid and galacturonic acid were detected and separated within 8 min. The relationship between the peak areas and the amounts of ABEE-derivatized monosaccharides was linear in the range of 1 to 1000 pmol.