- 著者
- Takegawa Yasuhiro Deguchi Kisaburo Keira Takuro Ito Hiroki Nakagawa Hiroaki Nishimura Shin-Ichiro
- 出版者
- Elsevier
- 雑誌
- Journal of Chromatography A (ISSN:00219673)
- 巻号頁・発行日
- vol.1113, no.1-2, pp.177-181, 2006-04-28
- 被引用文献数
- 114
Isomeric oligosaccharides and isomeric glycopeptides are sometimes difficult to separate on normal-phase (NP) and reversed-phase (RP) columns. A zwitterionic type of hydrophilic-interaction chromatography column with sulfobetaine groups (called ZIC-HILIC column) was first applied to the separation of 2-aminopyridine derivatized (PA) N-glycans and tryptic peptides of human serum immunoglobulin G (IgG). It is shown that the ZIC-HILIC column has high capability for structural recognition of isomeric N-glycans as well as high selectivity for glycopeptides. The former feature (i.e., structural recognition) was proven by sufficient separation of neutral PA N-glycan isomers, which are usually difficult to separate on NP and RP columns. In addition, it is noteworthy that IgG glycopeptides consisting of isomeric N-glycans and the same peptide sequences can be sufficiently separated on a ZIC-HILIC column. The latter feature (i.e., selectivity) was also demonstrated by easily separating two peptide groups with/without N-glycans. Thus, we note that the ZIC-HILIC column is highly promising for a simple analysis of N-glycans and N-glycopeptide samples. (c) 2006 Elsevier B.V. All rights reserved.