- 著者
- Shuai Zhou Manae Ninoseki Asahi Kusaba Kazuma Nakagawa Taro Urase
- 出版者
- Japan Society on Water Environment
- 雑誌
- Journal of Water and Environment Technology (ISSN:13482165)
- 巻号頁・発行日
- vol.19, no.6, pp.294-301, 2021 (Released:2021-12-10)
- 参考文献数
- 33
- 被引用文献数
- 1
Membrane bioreactor-reverse osmosis process (MBR-RO) is widely applied in wastewater treatment, especially when high-quality treated water is needed. Although MBR shows better performances in the removal of pathogens than the conventional activated sludge process, stable operation of RO process in the latter step of MBR-RO depends on residual bacteria in the filtrate of microfiltration membranes. Species and sizes of bacteria found in the filtrate of activated sludge with 0.2 μm pore-size polycarbonate membranes were investigated in this study. Isolated bacterial species grown on R2A agar medium were identified based on the full length 16S rRNA gene sequences. The result showed that approximately 90% of the isolates found in the filtrates were members of phylum Proteobacteria in which Ralstonia spp., Achromobacter spp., Methylobacterium spp. and Methylorubrum spp. accounted for the largest proportions while other bacteria affiliated with phyla Actinobacteria and Firmicutes. The leakage of Ralstonia and Actinobacteria was probably due to their small sizes judging from microscopic observation, while a longer filtration time is needed for Methylobacterium and Methylorubrum to be detected in the filtrate.
- 著者
- Hirofumi Tsutsui Taro Urase
- 出版者
- Japan Society on Water Environment
- 雑誌
- Journal of Water and Environment Technology (ISSN:13482165)
- 巻号頁・発行日
- vol.17, no.3, pp.194-202, 2019 (Released:2019-06-10)
- 参考文献数
- 21
- 被引用文献数
- 3
16S rRNA gene copy numbers and bacterial counts in the permeate from different microfiltration membranes installed in membrane bioreactors (MBRs) were measured in order to provide information on the selection of suitable membrane for wastewater reclamation and reuse. 16S rRNA gene copy numbers in the MBR effluent (105 − 107 gene copies / mL) were 1/5 to 1/500 of those in the centrifugal supernatant of the mixed liquor. High bacterial counts and high gene copies were observed in the permeate of the membranes at the beginning of the filtration stage. The permeate contained bacteria without exceptions by using a 0.4 µm or larger pore size membrane, although the passage of bacteria was sporadic by the use of a membrane with cylindrical pores of 0.2 µm diameter. Acinetobacter junii and Microbacterium fluvii were identified in the permeate of the 0.2 µm pore size membrane based on the closest match of 16S rRNA gene sequence. The possibility of selective passage of small size bacteria in the mixed liquor of activated sludge through the membrane was suggested.