- 著者
- Thi Huyen Do Ngoc Giang Le Trong Khoa Dao Thi Mai Phuong Nguyen Tung Lam Le Han Ly Luu Khanh Hoang Viet Nguyen Van Lam Nguyen Lan Anh Le Thu Nguyet Phung Nico M. van Straalen Dick Roelofs Nam Hai Truong
- 出版者
- Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
- 雑誌
- The Journal of General and Applied Microbiology (ISSN:00221260)
- 巻号頁・発行日
- pp.2017.08.004, (Released:2018-03-12)
- 参考文献数
- 55
- 被引用文献数
- 11
The scarcity of enzymes having an optimal activity in lignocellulose deconstruction is an obstacle for industrial-scale conversion of cellulosic biomass into biofuels. With the aim of mining novel lignocellulolytic enzymes, a ~9 Gb metagenome of bacteria in Vietnamese native goats’ rumen was sequenced by Illumina platform. From the data, 821 ORFs encoding carbohydrate esterases (CEs) and polysaccharide lyases (PLs) serving for lignocellulose pre-treatment, 816 ORFs encoding 11 glycoside hydrolase families (GHs) of cellulases, and 2252 ORFs encoding 22 GHs of hemicellulases, were mined. The carbohydrate binding module (CBM) was also abundant with 763 ORFs, of which 480 ORFs are located with lignocellulolytic enzymes. The enzyme modularity analysis showed that CBMs are usually present in endoglucanase, endo 1,3-beta-D-glucosidase, and endoxylanase, whereas fibronectin 3-like module (FN3) mainly represents in GH3 and immunoglobulin-like domain (Ig) was located in GH9 only. Every domain located in each ORF was analyzed in detail to contribute enzymes’ modularity which is valuable for modelling, to study the structure, and for recombinant production. With the aim of confirming the annotated results, a mined ORF encoding CBM63 was highly expressed in E. coli in soluble form. The purified recombinant CBM63 exhibited no cellulase activity, but enhanced a commercial cellulase activity in the destruction of a paper filter.