- 著者
- Tsuneko OKAZAKI
- 出版者
- The Japan Academy
- 雑誌
- Proceedings of the Japan Academy, Series B (ISSN:03862208)
- 巻号頁・発行日
- vol.93, no.5, pp.322-338, 2017-05-11 (Released:2017-05-11)
- 参考文献数
- 43
- 被引用文献数
- 5 9
At DNA replication forks, the overall growth of the antiparallel two daughter DNA chains appears to occur 5′-to-3′ direction in the leading-strand and 3′-to-5′ direction in the lagging-strand using enzyme system only able to elongate 5′-to-3′ direction, and I describe in this review how we have analyzed and proved the lagging strand multistep synthesis reactions, called Discontinuous Replication Mechanism, which involve short RNA primer synthesis, primer-dependent short DNA chains (Okazaki fragments) synthesis, primer removal from the Okazaki fragments and gap filling between Okazaki fragments by RNase H and DNA polymerase I, and long lagging strand formation by joining between Okazaki fragments with DNA ligase.