- 著者
- Xia JIANG Qian REN Shi-Ping CHEN Xiao-Bo TONG Mei DONG Shigeru SUGAYA Takeshi TANAKA Kazuko KITA Nobuo SUZUKI
- 出版者
- (社)日本農芸化学会
- 雑誌
- Bioscience, Biotechnology, and Biochemistry (ISSN:09168451)
- 巻号頁・発行日
- vol.75, no.9, pp.1685-1691, 2011-09-23 (Released:2011-09-23)
- 参考文献数
- 37
- 被引用文献数
- 6 1
Little is known about the ability of miso, to modulate mutability in human cells. We have observed increased levels of glucose-regulated protein 78 (GRP78) expression in association with suppression of mutation in human RSa cells irradiated with ultraviolet C (UVC). Here we examined to determine whether miso treatment results in increased GRP78 expression and suppression of UVC mutagenicity in RSa cells. Supernatants of water extracts of miso products and their components were tested. In the sample-treated cells, the amount of GRP78, as estimated by RT-PCR and immunoblotting analysis, increased, and the UVC-induced ouabain resistant mutation (OuaR) and the K-ras codon 12-base substitution mutation frequency decreased. This decrease was not observed in cells with downregulation of GRP78 by GRP78 siRNA transfection. The results suggest that miso suppresses UVC mutagenicity by increasing GRP78 expression in human cells.