著者

Shiga Takeki Suzuki Hiroyuki Yamamoto Ayumi YAMAMOTO Hiroaki YAMAMOTO Kazuo

出版者

Journal of Radiation Research Editorial Committee

雑誌

Journal of radiation research (ISSN:04493060)

巻号頁・発行日

vol.51, no.4, pp.405-415, 2010-07-16

被引用文献数

1 12

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Previously, we have shown that phenyl hydroquinone, a hepatic metabolite of the Ames test-negative carcinogen <i>o</i>-phenylphenol, efficiently induced aneuploidy in <i>Saccharomyces cerevisiae</i> by arresting the cell cycle at the G2/M transition as a result of the activation of the Hog1 (p38 MAPK homolog)-Swe1 (Wee1 homolog) pathway. In this experiment, we examined the aneuploidy forming effects of hydroquinone, a benzene metabolite, since both phenyl hydroquinone and hydroquinone are Ames-test negative carcinogens and share similar molecular structures. As was seen in phenyl hydroquinone, hydroquinone induced aneuploidy in yeast by delaying the cell cycle at the G2/M transition. Deficiencies in <i>SWE1</i> and <i>HOG1</i> abolished the hydroquinone-induced delay at the G2/M transition and aneuploidy formation. Furthermore, Hog1 was phosphorylated by hydroquinone, which may stabilize Swe1. These data indicate that the hydroquinone-induced G2/M transition checkpoint, which is activated by the Hog1-Swe1 pathway, plays a role in the formation of aneuploidy.

著者

Kudo Toshiyuki Yamamoto Hiroaki Sato Seiji SUTOU Shizuyo

出版者

THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

雑誌

The Journal of reproduction and development (ISSN:09168818)

巻号頁・発行日

vol.42, no.2, pp.101-107, 1996-05-01

参考文献数

28

被引用文献数

1 10

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Aromatase (EC 1.14.14.1.) is a key enzyme of feminizing hormone biosynthesis and catalyzes the conversion of androgens to estrogens. In birds, sex-steroid hormones, especially estrogens, play a critical role in the development of the gonadal glands and the aromatase is one of the most important factors in sex determination. Herein, we cloned the 5' upstream regions of chicken and quail aromatase genes and determined these sequences, which showed high homology between chicken and quail. Moreover, the transcription initiation site of the chicken aromatase gene in early development was determined by the 5'-RACE method. The findings showed that the transcription of the chicken aromatase gene starts from a more upstream site than previously reported.