著者

NORIYUKI FUKUNAGA MASATO WADA MAMORU HONJO YUHKO SETAISHI NAOKI HAYASHINAKA YASUHIRO TAKADA JIRO NISHIKAWA

出版者

公益財団法人 応用微生物学・分子細胞生物学研究奨励会

雑誌

The Journal of General and Applied Microbiology (ISSN:00221260)

巻号頁・発行日

vol.41, no.3, pp.191-205, 1995 (Released:2006-08-22)

参考文献数

35

被引用文献数

1 10

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A Gram-negative, aerobic bacterium isolated from the bottom layer of Lake Vanda, Antarctica, can grow in a synthetic medium containing biotin as a growth factor. The lipid and fatty acid compositions of the cells grown in the fatty acid-free medium were analyzed. Phosphatidylethanolamine, phosphatidylglycerol and wax esters were identified as major components of the chloroform-methanol extractable lipids. Palmitoleic and oleic acids comprised more than 90% of the total fatty acid of the lipids when the bacterium was grown at low temperature (5°C). Increasing the growth temperature induced an increase in the average fatty acyl chain length of the phospholipids and a decrease in the unsaturation degree of the fatty acyl chain of the wax esters. Increasing the NaCl concentration in the medium also induced an increase in the average fatty acyl chain length of the phospholipids. The effects of temperature and NaCl were compared further at the level of phospholipid molecular species. The sn-2 position of phosphatidylethanolamine and the sn-1 position of phosphatidylglycerol were the most sensitive sites to changes of temperature and NaCl concentration, respectively. An aerobic desaturase system was demonstrated to be the only pathway for unsaturated fatty acid synthesis in this bacterium.