著者
Kenji Fujino Yoshihiro Kawahara Kanako O. Koyanagi Kenta Shirasawa
出版者
Japanese Society of Breeding
雑誌
Breeding Science (ISSN:13447610)
巻号頁・発行日
pp.20089, (Released:2021-02-17)
被引用文献数
8

Understanding genetic diversity among local populations is a primary goal of modern crop breeding programs. Here, we demonstrated the genetic relationships of rice varieties in Hokkaido, Japan, one of the northern limits of rice cultivation around the world. Furthermore, artificial selection during rice breeding programs has been characterized using genome sequences. We utilized 8,565 single nucleotide polymorphisms and insertion/deletion markers distributed across the genome in genotype-by-sequencing for genetic diversity analyses. Phylogenetics, genetic population structure, and principal component analysis showed that a total of 110 varieties were classified into four distinct clusters according to different populations geographically and historically. Furthermore, the genome sequences of 19 rice varieties along with historic representations in Hokkaido, nucleotide diversity and FST values in each cluster revealed that artificial selection of elite phenotypes focused on chromosomal regions. These results clearly demonstrated the history of the selections on agronomic traits as genome sequences among current rice varieties from Hokkaido.
著者
Yoshihiro Kawahara Tomoko Endo Mitsuo Omura Yumiko Teramoto Takeshi Itoh Hiroshi Fujii Takehiko Shimada
出版者
Japanese Society of Breeding
雑誌
Breeding Science (ISSN:13447610)
巻号頁・発行日
pp.19097, (Released:2020-03-05)
被引用文献数
10

Citrus species are some of the most valuable and widely consumed fruits globally. The genome sequences of representative citrus (e.g., Citrus clementina, C. sinensis, C. grandis) species have been released but the research base for mandarin molecular breeding is still poor. We assembled the genomes of Citrus unshiu and Poncirus trifoliata, two important species for citrus industry in Japan, using hybrid de novo assembly of Illumina and PacBio sequence data, and developed the Mikan Genome Database (MiGD). The assembled genome sizes of C. unshiu and P. trifoliata are 346 and 292 Mb, respectively, similar to those of citrus species in public databases; they are predicted to possess 41,489 and 34,333 protein-coding genes in their draft genome sequences, with 9,642 and 8,377 specific genes when compared to C. clementina, respectively. MiGD is an integrated database of genome annotation, genetic diversity, and Cleaved Amplified Polymorphic Sequence (CAPS) marker information, with these contents being mutually linked by genes. MiGD facilitates access to genome sequences of interest from previously reported linkage maps through CAPS markers and obtains polymorphism information through the multiple genome browser TASUKE. The genomic resources in MiGD (https://mikan.dna.affrc.go.jp) could provide valuable information for mandarin molecular breeding in Japan.
著者
Wei Wei Akihiro Nakamata Yoshihiro Kawahara Tohru Asami
出版者
一般社団法人 情報処理学会
雑誌
Journal of Information Processing (ISSN:18826652)
巻号頁・発行日
vol.23, no.6, pp.835-844, 2015 (Released:2015-11-15)
参考文献数
17
被引用文献数
4

In this paper, we demonstrate a food recognition method by monitoring power leakage from a domestic microwave oven. Universal Software Radio Peripheral (USRP) is applied as a low-cost spectrum analyzer to measure the microwave oven leakage as received signal strength indication (RSSI). We aim to recognize 18 categories of food that are commonly cooked in a microwave oven. By analyzing 180 features that contain the information of heating-time difference, we attain an average recognition accuracy of 82.3%. Using 138 features excluding the heating-time difference information, the average recognition accuracy is 56.2%. The recognition accuracy under different conditions is also investigated, for instance, utilizing different microwave ovens, different distances between the microwave oven and the USRP as well as different data down-sampling rates. Finally, a food recognition application is implemented to demonstrate our method.