- 著者
- Yoshio Kimura Sayaka Kajimoto Yuuka Yamamoto Naotaka Tanaka
- 出版者
- Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
- 雑誌
- The Journal of General and Applied Microbiology (ISSN:00221260)
- 巻号頁・発行日
- vol.66, no.1, pp.46-50, 2020 (Released:2020-04-13)
- 参考文献数
- 22
- 被引用文献数
- 1 3
Myxococcus xanthus Nudix hydrolase 2 (Nud2) hydrolyzed oxidized deoxynucleotides, such as 8-oxo-dGTP, 8-oxo-dGDP, 8-OH-dTP, and 2-OH-dATP, and showed the highest specific activity toward 8-oxo-dGTP. Mn2+ was the most effective co-factor for stimulating oxidized deoxynucleotide hydrolase activity. The Km of Nud2 with 8-oxo-dGTP for Mn2+ was 19-fold lower than that for Mg2+, and was 2-fold lower than that with dGTP for Mn2+. The specificity constant (kcat/Km) for 8-oxo-dGTP was 6-fold higher than that for dGTP. Nud2 contains a similar Nudix motif (84AX590GX7REX2EEXGX). Replacement of Ala84 and/or Gly90 in the Nudix motif of Nud2 by Gly or Glu had negligible effects on 8-oxo-dGTP hydrolase activity, suggesting that a strict Nudix motif sequence is not essential for complete hydrolase activity of Nud2.