- 著者
- Zhao-qiang Zhang Xiao Sun Xiang-lin Chi Xian-chang Sun Hong Jiang
- 出版者
- Japan Brain Science society
- 雑誌
- 脳科学誌 (ISSN:13415301)
- 巻号頁・発行日
- vol.40, pp.54-65, 2013-03-30 (Released:2017-06-01)
- 参考文献数
- 11
Objective Modeling a stable and reproducible animal model of Parkinson's disease by rotenone intraperitoneal injection for further pathogenesis study of PD. Methods Twenty-seven male Wistar rats were used, which were randomly divided into three groups on average (Rotenone Injection, Vehicle Injection and Normal group). Rats of rotenone injection group (RIG) were administered rotenone (3.0 mg/kg/day) in a specialized vehicle through daily intraperitoneal injection; rats of vehicle injection group (VIG) were administered only special vehicle in the same way, rats of normal group (NG) didn't receive any injection. Checked the changes of its behavior and numbers of SNpc neurons to determine the model successful or not. Results Six rats of RIG developed part of Parkinson's symptoms at different time. All rats of RIG emerged behavioral deficits through rearing behavior testing and square bridge testing, and the mean number of SNpc neurons showed significant reduction. There were no behavioral deficits and changes of the mean number of SNpc neurons in rats of NG and VIG. Conclusion Model of Parkinson's disease by rotenone intraperitoneal injection was successfully established. This form of the rotenone model is stable and easy to reproduce, and may provide a new excellent supporter to related studies.
- 著者
- Zhao-qiang Zhang Xiao Sun Bing-xiang Wang Yan-ling Zhang
- 出版者
- Japan Brain Science society
- 雑誌
- 脳科学誌 (ISSN:13415301)
- 巻号頁・発行日
- vol.36, pp.50-60, 2011-03-30 (Released:2017-06-01)
- 参考文献数
- 10
Objective Modeling a surgery hypertensive rat model, observe the changes of the expression of ACE and ACE2 in various groups, thus for further research of the pathogenesis of hypertension. Methods Twenty-eight Sprague- Dawley rats were used, which were divided randomly into four groups. One group served as control (n=7), others as hypertensive group (n=7), enalapril group [n=7,15mg/(kg・d)] and losartan group [n=7,15mg/(kg・d)]. All rats were operated as surgery hypertension except rats of control group. Renal artery was ligated. Enalapril and losartan were used as treatments of rats of enalapril group and losartan group after surgery. Blood pressure of rats was measured by arteria caudilis uninjured. The expression of renal ACE and ACE2 mRNA were measured by RT-PCR. The expression of renal ACE and ACE2 protein were measured by immunohistochemistry. Results Blood pressure of rats was increased after operation. The expression of ACE and ACE2 mRNA and protein in various groups were different. The expression of ACE mRNA and protein of Hypertensive group were higher than others. The expression of ACE2 mRNA and protein of losartan group were higher than others. Conclusion Model of surgery hypertensive rats were success by the ligation of renal artery. It's a Simple, reliable and cheap method of hypertensive rat model. The imbalance between the expression of ACE and ACE2 is one of the important roles of pathogenesis of hypertension.