- 著者
- Yasuyoshi Kurimoto Yasuyo Shimomura Kazuhiro Moriyama Tomoyuki Nakamura Naohide Kuriyama Yoshitaka Hara Hidefumi Komura Daisuke Hasegawa Takahiro Kawaji Osamu Nishida
- 出版者
- 藤田学園医学会
- 雑誌
- Fujita Medical Journal (ISSN:21897247)
- 巻号頁・発行日
- pp.2019-002, (Released:2019-11-02)
- 参考文献数
- 11
Objective: Sivelestat sodium hydrate (Siv) is expected to be an effective therapy for acute respiratory distress syndrome, although its mechanism of action is not understood. In this study, we investigated which myeloid cells-derived cytokines were suppressed by Siv.Methods: Continuous hemofiltration was performed by circulating fresh porcine blood through a semi-closed circuit. To ensure that leukocytes survived for 360 min, 5% glucose, heparin, and air were continuously injected. The control group received continuous administration of lipopolysaccharide (LPS) only, whereas the Siv group received LPS and Siv. Complete blood count, levels of various cytokines, and other variables were compared between the groups.Results: Interleukin (IL)-1β level was significantly suppressed in the Siv group compared with that in the control group (p<0.05).Conclusions: The results suggested that Siv suppressed the production of IL-1β and possibly other cytokines by myeloid cells. Whether this suppression of cytokine production is caused directly by Siv or mediated via suppression of granulocyte elastase should be evaluated in the future.
- 著者
- Takumi Kito Kazuhiro Nishii Runhong Yao Toshio Teranishi Tomohisa Sugiyama Kazuyoshi Sakai Mamoru Matsubara Kouji Yamada
- 出版者
- Fujita Medical Society
- 雑誌
- Fujita Medical Journal (ISSN:21897247)
- 巻号頁・発行日
- vol.5, no.3, pp.57-62, 2019 (Released:2019-08-01)
- 参考文献数
- 21
Objectives: Bone fractures affect the activities of daily living and lower quality of life, so investigating preventative measures is important. We developed novel stimulation equipment that combined a vibration stimulus with a shaking stimulus for preventing osteoporosis (one of the causes of bone fractures). We aimed to investigate the effect of this equipment on ovariectomized mice.Methods: Oophorectomy of 8-week-old female mice was done. The stimulation group was stimulated for 10 consecutive weeks.Results: The stimulation group showed significantly higher values (p<0.05) for osteoid thickness, osteoid volume-to-bone volume ratio and mineral apposition rate than those in the non-stimulation control group. The stimulation group showed significantly higher values (p<0.05) compared with the non-stimulation for expression of bone morphogenetic protein-2, interleukin-1β, interleukin-6 and myogenic determination gene in quadriceps femoris muscles (QFMs).Conclusions: These data suggest that cytokine secretion by QFMs carried a humoral factor throughout the body via the blood and blood vessels and acted on bone and various organs. Development of this stimulation method and its clinical application, new methods for preventing and treating osteoporosis could ensue.