著者

Kakui Keiichi Hiruta Chizue

出版者

John Wiley & Sons

雑誌

Journal of Morphology (ISSN:03622525)

巻号頁・発行日

vol.275, no.9, pp.1041-1052, 2014-09

被引用文献数

14

---

Among arthropods, various insects, spiders, and crustaceans produce thread. The crustacean Tanaidacea include species that use thread mainly to construct dwelling tubes. While thread production was previously known only in Tanaoidea and Paratanaoidea, it was recently discovered in two species in Kalliapseudidae (Apseudoidea), although information on the morphology of the thread‐producing system was lacking. Using histology, light and scanning electron microscopy, we found that the kalliapseudid Phoxokalliapseudes tomiokaensis comb. nov. lacks the sort of glandular structures associated with thread production in the pereonites, but has these structures in pereopods 1–6. We observed four types of glandular systems defined by the types and distribution of glands they contain: Type A (pereopod 1), Type B (pereopods 2 and 3), Type C (pereopods 4 and 5), and Type D (pereopod 6). All types have small rosette glands and lobed glands; Type A additionally has large rosette glands. The inferred thread‐producing apparatus in P. tomiokaensis is very different from that in Tanaoidea and Paratanaoidea, suggesting that kalliapseudids evolved thread production independently from the latter two groups. J. Morphol. 275:1041–1052, 2014. © 2014 Wiley Periodicals, Inc.

著者

Akita Hidetaka Ito Rie Kamiya Hiroyuki Kogure Kentaro Harashima Hideyoshi

出版者

John Wiley & Sons

雑誌

The Journal of Gene Medicine (ISSN:1099498X)

巻号頁・発行日

vol.9, no.3, pp.197-207, 2007-03

被引用文献数

36

---

Background: Heterogeneity of transgene expression, the presence or absence (below the limit of detection) of transgene expression on a cell-by-cell basis, is a severe disadvantage in the use of cationic lipid-mediated gene vectors for gene therapy and experiments in molecular biology. Understandings of intracellular trafficking and the function (transgene expression) of vectors related to cellular physiology are essential in terms of clarifying the mechanism underlying the heterogeneity. Methods: To distinguish the contribution of nuclear transfer efficiency and subsequent intranuclear transcription efficiency to the overall heterogeneity in transgene expression, a novel imaging system was established for the dual visualization of the nuclear transfer of pDNA and marker gene expression (lacZ) in single cells. Results: The expression of LacZ occurred in only approximately 30% of HeLa cells of the nuclear pDNA-positive cells, indicating that intranuclear transcription efficiency contributed to the heterogeneity. Dual imaging against synchronized cells further revealed that the efficiency of nuclear delivery was comparable irrespective of cell cycle status, which is contrary to the generally accepted hypothesis that nuclear import of pDNA is enhanced during cell division when the nuclear membrane structure is perturbed. The most significant finding in the present study is that nuclear transcription efficiency in terms of the ratio of LacZ-positive cells to nuclear pDNA-positive cells drastically increased in the late S and G2/M phase. Conclusions: This is the first demonstration to show that cell cycle dependent intranuclear transcription appears to be responsible for the overall heterogeneity of transgene expression. Copyright © 2007 John Wiley & Sons, Ltd.

著者

Natsuga Ken Furuta Yoshikazu Takashima Shota Nohara Takuma Kosumi Hideyuki Mai Yosuke Higashi Hideaki Ujiie Hideyuki

出版者

John Wiley & Sons

雑誌

Human mutation (ISSN:10597794)

巻号頁・発行日

vol.43, no.4, pp.529-536, 2022-04-01

被引用文献数

4

---

Revertant mosaicism (RM) is a phenomenon in which inherited mutations are spontaneously corrected in somatic cells. RM occurs in some congenital skin diseases, but genetic validation of RM in clinically revertant skin has been challenging, especially when homologous recombination (HR) is responsible for RM. Here, we introduce nanopore Cas9-targeted sequencing (nCATS) for identifying HR in clinically revertant skin. We took advantage of compound heterozygous COL7A1 mutations in a patient with recessive dystrophic epidermolysis bullosa who showed revertant skin spots. Cas9-mediated enrichment of genomic DNA (gDNA) covering the two mutation sites (>8 kb) in COL7A1 and subsequent MinION sequencing successfully detected intragenic crossover in the epidermis of the clinically revertant skin. This method enables the discernment of haplotypes of up to a few tens of kilobases of gDNA. Moreover, it is devoid of polymerase chain reaction amplification, which can technically induce recombination. We, therefore, propose that nCATS is a powerful tool for understanding complicated gene modifications, including RM.

著者

Ikenaka Yoshinori Miyabara Yuichi Ichise Takahiro Nakayama Shouta Nimako Collins Ishizuka Mayumi Tohyama Chiharu

出版者

John Wiley & Sons

雑誌

Environmental toxicology and chemistry (ISSN:07307268)

巻号頁・発行日

vol.38, no.1, pp.71-79, 2019-01

被引用文献数

57

---

Neonicotinoid insecticides that have been on the market since 1992 have been used globally including in Japan. Because they are sprayed over forests and agricultural areas, inadvertent toxicity in nontarget insects (especially honey bees) and humans is a matter of public concern. However, information on exposure levels and potential health impacts of neonicotinoids in children living around sprayed areas is scarce. Thus, we determined neonicotinoid exposure levels in children living in communities where thiacloprid was used to control pine wilt disease. A total of 46 children (23 males and 23 females) were recruited for the present study, and informed written consent was obtained from their guardians. Urine specimens were collected before, during, and after insecticide spraying events; and atmospheric particulate matter was also collected. Concentrations of thiacloprid and 6 other neonicotinoid compounds were determined in urine samples and in atmospheric particulate matter specimens using liquid chromatography-electrospray ionization-tandem mass spectrometry. In urine specimens, thiacloprid concentrations were <0.13 mu g/L and were detectable in approximately 30% of all samples. Concentrations of the other neonicotinoids, N-dm-acetamiprid, thiamethoxam, dinotefuran, and clothianidin, were 18.7, 1.92, 72.3, and 6.02 mu g/L, respectively. Estimated daily intakes of these neonicotinoids were then calculated from urinary levels; although the estimated daily intakes of the neonicotinoids were lower than current acceptable daily intake values, the children were found to be exposed to multiple neonicotinoids on a daily basis. Environ Toxicol Chem 2019;38:71-79. (c) 2018 SETAC