- 著者
- Iba Yoshinori
- 出版者
- Kinki University Medical Association
- 雑誌
- ACTA MEDICA KINKI UNIVERSITY = The Kinki University Medical Association (ISSN:03866092)
- 巻号頁・発行日
- vol.40, no.1, pp.7-14, 2015-06-01
<Abstract>Orthostatic dysregulation (OD), an autonomic imbalance manifesting dizziness upon standing up and vertigo as principal symptoms, is also considered to be a partly psychosomatic disorder because the condition is likely to be influenced by mental stress. Nephrotic syndrome (NS) can cause such stress in relation to recurrences, exacerbations, and vulnerability to infections. In this study we retrospectively investigated symptoms of depression in 7-to 15-year-old children with OD and NS using the Birleson Depression Self-Rating Scale for Children (DSRS-C). Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) was measured in NS patients as a urinary oxidative stress marker. In children with OD, DSRS-C scores were significantly higher than in a general population of elementary and junior high school students (P=0.00030 for elementary and junior high school students combined; P=0.0057 for junior high school students). In children with NS, DSRS-C scores were significantly lower than in the other elementary and junior high school students (P=0.019). Urinary 8-OHdG did not correlate significantly with the number of NS recurrences, duration of illness, or incidence of recurrence. No NS-related variable was found to be significantly correlated with oxidative stress. School absence often persists in OD patients even after somatic symptoms have been improved by drug therapy such as midodrine hydrochloride treatment, suggesting that symptoms of depression may prolong school absence in those with OD. Since the DSRS-C can rapidly quantify the severity of depression, early administration is recommended for children with OD.
- 著者
- Masaki Hideyuki
- 出版者
- Kinki University Medical Association
- 雑誌
- ACTA MEDICA KINKI UNIVERSITY = The Kinki University Medical Association (ISSN:03866092)
- 巻号頁・発行日
- vol.38, no.2, pp.101-109, 2013-12-01
[Abstract] Xenograft rejection caused by xenoreactive T cells is a major obstacle to xenotransplantation. To analyze anti-xeno T-cell responses, xeno-reactive T-cell clones derived from C57BL/6 (B6) mice immunized with Wistar Furth (WF) rat cells were established. All CD8 clones proliferated in response to WF rat thymocytes even in the absence of B6 mouse splenocytes as antigen-presenting cells, indicating that they responded to xeno-antigen via the direct recognition pathway. In contrast, all CD4 clones proliferated against WF cells only when syngeneic splenocytes were added to the cultures, indicating that they responded via the indirect recognition pathway. All CD8 clones proliferated in response to thymocytes from WF, BB, or PVG.1R8 rats, but not to LEWIS or PVG rats. They also lysed NK-resistant C58NT(D) lymphoma cells of WF rat origin in a dosedependent manner, suggesting specificity for the rat MHC class I RT1C" allele, and their function as cytotoxic T lymphocytes. All CD4 clones proliferated against rat thymocytes of various strains only in the presence of splenocytes from B6 or BALB.B mice, which possess the H-2b haplotype, indicating that they recognize rat xeno-antigens shared between different strains in an H-2"-restricted manner. All CD4 clones secreted predominantly interferon y in response to rat xeno-antigen, suggesting that they possess Thl characteristics. Because all clones are likely to act as effector cells for xenograftrejection, they are expected to be useful for investigating mechanisms of xenograft rejection mediated by cellular immunity, and should be a good source of TcR genes for creating transgenic mice to supply naive xeno-reactive T cells.