著者

Yoh-ichi Watanabe Shigeo Yoshinari

出版者

The Society for the Study of the Origin and Evolution of Life Japan

雑誌

Viva Origino (ISSN:09104003)

巻号頁・発行日

vol.41, no.2, pp.12-13, 2013 (Released:2021-12-29)

参考文献数

23

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In Archaea, almost all introns in pre-tRNA, pre-rRNA, and pre-mRNA are spliced through two common steps by protein enzymes: cleavage of the precursor with splicing endonuclease, and ligation of the exons with RNA ligase. We found the first examples of archaeal pre-mRNA splicing and cleavage of the pre-mRNA with a novel subclass of archaeal splicing endonuclease. We further solved the novel tertiary structure of the splicing endonuclease, and revealed that the lineage-specific insertion of amino acid residues in the endonuclease expands the recognition of the substrate precursor RNA. We also discuss the possible involvement of tRNA splicing and its machinery in the origin of the tRNA molecule.