Afternoon literature rabbit hole… Found this work showing that EGFP-ß-actin (Cter fusion) is better than the widely used ß-actin-EGFP (Nter fusion): https://t.co/kgDcqJrEl6
However this is not mentioned in reviews such as https://t.co/q64SpkEKmu. Thoughts? https://t.co/KQK4KDnVkg
I've been looking for this pdf like forever, and it just popped up in a Google search. Tsukita & Ishikawa 1981 serial EM section study of axonal microtubules, with a real wire-built model https://t.co/HbM8WkhEG9 https://t.co/rfutGmmOsi
https://t.co/6BP3DGL5I7
Sakaue-Sawano and Miyawaki have published a paper describing a new cell cycle biosensor, Fucci5. In addition to the improved sensitivity, the new Fucci is free from the patents of fluorescent proteins, paving the way to the commercial use!