著者

TAYAMA Yoshitaka MORIYASU Aya SUGIHARA Kazumi OHTA Shigeru KITAMURA Shigeyuki

出版者

The Japanese Society for the Study of Xenobiotics

雑誌

Drug metabolism and pharmacokinetics (ISSN:13474367)

巻号頁・発行日

vol.22, no.2, pp.119-124, 2007-04-25

参考文献数

41

被引用文献数

12

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In this study, the developmental changes and variability of aldehyde oxidase in postnatal rat liver were examined. Postnatal day 1, 7 and 14 rats showed little or no liver aldehyde oxidase activity, as evaluated in terms of the activities for oxidation of benzaldehyde to benzoic acid, <i>N</i>-1-methylnicotinamide (NMN) to <i>N</i>-1-methyl-2-pyridone-5-carboxamide (2-PY) and <i>N</i>-1-methyl-4-pyridone-3-carboxamide (4-PY), and methotrexate (MTX) to 7-hydroxymethotrexate (7-OH-MTX). However, these oxidase activities were markedly increased in liver cytosol from the rats after postnatal day 14. The activity was then maintained up to 6 weeks. The amounts of 2-PY and 4-PY formed from NMN were almost the same. The development of aldehyde oxidase activity toward benzaldehyde was closely correlated with that of oxidase activity toward NMN and MTX. The expression of aldehyde oxidase at postnatal day 14 was confirmed by Western blotting analysis. The density of bands of aldehyde oxidase was closely correlated with the oxidase activity toward benzaldehyde. The developmental changes of aldehyde oxidase activities during postnatal reflected the changes in the amount of the oxidase protein. Thus, aldehyde oxidase activity in rats rapidly increases from birth, reaching a plateau within 4 weeks, and is regulated by expression of the protein.<br>