著者
KAWABATA Yasuyuki KUNO Atsushi GAMA Yasuo KUSAKABE Isao
出版者
社団法人日本農芸化学会
雑誌
Bioscience, biotechnology, and biochemistry (ISSN:09168451)
巻号頁・発行日
vol.60, no.3, pp.524-525, 1996-03-23
被引用文献数
1 5

The substrate speciticity of a p-nitrophenyl α-D-glucopyranosyl-uronic acid-hydrolyzinig enzyme (PNP-GAase) isolated from snail acetone powder has been investigated with various substrates, such as P-nitrophenyl α-D-glucopyranosyluronic acid (PNP-GA), 2-O-α-D-glucopyraniosyluronic acid-D-xylose (GA-2X), 2-O-(4-O-methyl-α-D-glucopyranosyluronic acid)-D-xylose (MeGA-2X), and O-α-D-glucopyranosyluronic acid-α-D-glucopyranosiduronic acid (GA-GA). The K_m (mM) and V_<max> (μmol of glucuronic acid formed/mg of en-zyme protein/min) toward these substrates were as follows; 0.13 and 3.21 for PNP-GA, 0.33 and 0.089 for GA-2X, 17.6 and 0.094 for MeGA-2X, and 0.36 and 0.015 for GA-GA, respectively. The results indicate that the PNP-GAase specifically hydrolyzed PNP-GA, however, the enzyme had broad substrate specificity.

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こんな論文どうですか? Substrate Specificity of α-Glucuronidase Isolated from Snail Acetone Powder(KAWABATA Yasuyukiほか),1996 … https://t.co/hvwy6jrTG8
こんな論文どうですか? Substrate Specificity of α-Glucuronidase Isolated from Snail Acetone Powder(KAWABATA Yasuyukiほか),1996 … http://t.co/hvwy6jK2Ug

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