- 著者
- KAWABATA Yasuyuki KUNO Atsushi GAMA Yasuo KUSAKABE Isao
- 出版者
- 社団法人日本農芸化学会
- 雑誌
- Bioscience, biotechnology, and biochemistry (ISSN:09168451)
- 巻号頁・発行日
- vol.60, no.3, pp.524-525, 1996-03-23
- 被引用文献数
- 1 5
The substrate speciticity of a p-nitrophenyl α-D-glucopyranosyl-uronic acid-hydrolyzinig enzyme (PNP-GAase) isolated from snail acetone powder has been investigated with various substrates, such as P-nitrophenyl α-D-glucopyranosyluronic acid (PNP-GA), 2-O-α-D-glucopyraniosyluronic acid-D-xylose (GA-2X), 2-O-(4-O-methyl-α-D-glucopyranosyluronic acid)-D-xylose (MeGA-2X), and O-α-D-glucopyranosyluronic acid-α-D-glucopyranosiduronic acid (GA-GA). The K_m (mM) and V_<max> (μmol of glucuronic acid formed/mg of en-zyme protein/min) toward these substrates were as follows; 0.13 and 3.21 for PNP-GA, 0.33 and 0.089 for GA-2X, 17.6 and 0.094 for MeGA-2X, and 0.36 and 0.015 for GA-GA, respectively. The results indicate that the PNP-GAase specifically hydrolyzed PNP-GA, however, the enzyme had broad substrate specificity.
- 著者
- SHIMOKAWA Tomoko YOSHIDA Shigeki TAKEUCHI Toshio MURATA Katsumi ISHII Tadashi KUSAKABE Isao
- 出版者
- 社団法人日本農芸化学会
- 雑誌
- Bioscience, biotechnology, and biochemistry (ISSN:09168451)
- 巻号頁・発行日
- vol.60, no.9, pp.1532-1534, 1996-09-23
- 被引用文献数
- 4 36
The objective of this study was to prepare two series of authentic oligo-guluronic acids from sodium alginate. Oligo-guluronic acids (DP=1-9) were prepared from an acid hydrolysate of poly-guluronic acid by successive chromatographies of Bio-Gel P-6 and Q Sepharose Fast Flow. Oligo-guluronic acids having 4-deoxy-L-erythro-hex-4-enopyranosyluronic acid residues at the non-reducing end (DP=2-7) were prepared from the enzymatic degradation products of the poly-guluronic acid in the same manner. Each of the isolated oligo-guluronic acids gave a single band on fluorophore-assisted carbohydrate electrophoresis. These results suggest that successive chromatographies used in this study are well suited for the preparation of alginate-derived oligouronic acids.