著者

阿部 皓一 石橋 恭子 大前 雅彦 河部 靖 勝井 五一郎

出版者

日本ビタミン学会

雑誌

ビタミン (ISSN:0006386X)

巻号頁・発行日

vol.51, no.3, pp.111-117, 1977-03-25

被引用文献数

2

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A Method for the determination of ubiquinone homologues (UQ) in serum and liver is described. Serum or liver homogenate was saponified with methanolic alkaline solution after addition of 2,3,6-trimethyl-5-nonaprenyl-1,4-benzoquinone (TQ-9) as an internal standard and then extracted with n-hexane. The n-hexane extract was evaporated under N_2 gas at 30℃, and the residue was dissolved in dioxane. The sample solution was applied to the high-speed liquid chromatography (HSLC) using a Permaphase ODS column and ethanol・water (80 : 20) as a mobile phase. The peaks of UQ and TQ-9 were not disturbed by other fat-soluble substances when an on-line high-sensitive multiple wavelength detector set at 275 nm (absorption maximum of UQ) was used as a detector. The proposed HSLC method was found to be more simple and specific than the other conventional determination methods.