著者

Nishihara Tsutomu Nishikawa Jun-ichi Kanayama Tomohiko Dakeyama Fumi Saito Koichi Imagawa Masayoshi Takatori Satoshi Kitagawa Yoko Hori Shinjiro Utsumi Hideo

出版者

公益社団法人日本薬学会

雑誌

Journal of health science (ISSN:13449702)

巻号頁・発行日

vol.46, no.4, pp.282-298, 2000-08-01

被引用文献数

65

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One of the urgent tasks in understanding endocrine disruptors (EDs) is to compile a list of suspected substances among the huge number of chemicals by using the screening test method. We developed a simple and rapid screening method using the yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators. To date, we have tested the estrogenic activity of more than 500 chemicals including natural substances, medicines, pesticides, and industrial chemicals. 64 compounds were evaluated as positive, and most of these demonstrated a common structure; phenol with a hydrophobic moiety at the para-position without bulky groups at the ortho-position. These results are expected to facilitate further risk assessment of chemicals.