著者

平島 昭司 堀越 正美 中西 義信 平井 洋 関水 和久 水野 伝一 名取 俊二

出版者

公益社団法人日本薬学会

雑誌

Chemical & pharmaceutical bulletin (ISSN:00092363)

巻号頁・発行日

vol.35, no.2, pp.877-881, 1987-02-25

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We previously reported the purification of eukaryotic transcription factor S-II and its phosphorylated form S-H' from Ehrlich ascites tumor cells. In this work, we modified the previous purification procedure to make it quicker and simpler. By this improved procedure, involving a different solubilization method, and improved buffer solutions and centrifugation processes, S-II could be purified to homogeneity from 1 kg of frozen cells in about 10 d instead of 1 month. The purity, specific activity and total yield of S-II purified by the new method were comparable to those obtained by using the previous method.