著者

X. イリヤル 山本 豊 加藤 治文 荒明 美奈子 黒岩 ゆかり 會沢 勝夫

出版者

日レ医誌

雑誌

日本レーザー医学会誌 (ISSN:02886200)

巻号頁・発行日

vol.17, pp.281-284, 1996

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The cytotoxicity of PDT with ME2906 and Laser to P388 cells was examined. Cell viability was assayed by the MTT method. When the laser irradiated at 10J/cm<SUP>2</SUP> after incubation for 4 hours with 10&mu;g/ml of ME2906, cell viability was not decreased but with 20 and 30&mu;g/ml of ME2906, cell viabilities were 9 and 1.4% respectively. When the laser irradiated at 10J/cm<SUP>2</SUP> after incubation for 24 hours, cell viabilities were almost same as those of 4 hours incubation. ME2906 contents at 24 hours after drug administration at concentration of 10&mu;g/ml measured 2.8&times;10<SUP>-9</SUP>&mu;g/cell, and at 4 hours after drug administration at 20&mu;g/ml measured 5.5&times;10<SUP>-9</SUP>&mu;g/cell. These data showed that the cytotoxicity of PDT to P388 cells was dependent on the ME2906 intracellular accumulated contents but independent on laser doses.