著者

Satou Chiemi Goto Hirofumi Yamazaki Yuya Saitou Katsuyoshi Matsumoto Shoji Takahashi Ou Miyazaki Yosuke Ikuta Keiichi Yajima Yosuke

出版者

公益社団法人 日本油化学会

雑誌

日本油化学会誌 (ISSN:13458957)

巻号頁・発行日

vol.66, no.6, pp.601-606, 2017

被引用文献数

4

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<p>Monoacylglycerol (MAG) and diacylglycerol (DAG) are minor components of edible fats and oils, and they relate to the quality of these foods. The AOCS official method Cd 11b-91 has been used to determine MAG and DAG contents in fats and oils. There are, however, difficulties in the determination of MAG and DAG using this analytical procedure. Therefore, we improved this method by modifying the trimethylsilyl derivatization procedure and replacing the internal standard (IS) material. In our modified method, TMS-HT (mixture of hexamethyldisilazane and trimethylchlorosilane) was used for derivatization of MAG and DAG, which was followed by liquid-liquid extraction with water and <i>n</i>-hexane solution containing the IS, tricaprin. Using the modified method, we demonstrated superior repeatability in comparison with that of the AOCS method by reducing procedural difficulties. The relative standard deviation of distearin peak areas was 1.8% or 2.9% in the modified method, while it was 5.6% in the AOCS method. In addition, capillary columns, such as DB-1ht and DB-5ht could be used in this method.</p>