著者

上田 舞 高島 康弘

出版者

日本サイトメトリー学会

雑誌

サイトメトリーリサーチ (ISSN:09166920)

巻号頁・発行日

vol.27, no.1, pp.19-24, 2017-05-25 (Released:2017-05-31)

参考文献数

20

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Since mouse embryonic stem cells (ESCs) were established from pre-implantation epiblasts, mouse genomics has dramatically progressed and life science has been expanded. After the establishment of human ESCs in 1998 and human induced pluripotent stem cells (iPSCs) in 2007, there has been added expectation for regenerative medicine from pluripotent stem cells (PSCs). However, the pluripotency character of PSCs is not as well studied as the differentiation potential. We still do not know why, for example, mouse and human PSCs have distinct morphologies and signaling requirements for the maintenance of pluripotency as well as different transcriptome and epigenome.Currently, PSCs are classified into two pluripotent states, naïve and primed. Mouse PSCs represent naïve state, which corresponds to pre-implantation epiblasts and can contribute to chimaeras efficiently, whereas human PSCs represent primed state, which corresponds to post-implantation epiblasts.We have recently succeeded in generating human naïve PSCs by transient overexpression of NANOG and KLF2 in human primed PSCs. Our human naïve PSCs and mouse ESCs/iPSCs have similar gene expression pattern. Furthermore, they have DNA hypomethylation and alteration in mitochondrial metabolism that resemble preimplantation epiblasts. Human naïve PSCs may be useful to overcome the problems of heterogeneous populations, variation between cell lines, and the poorer differentiation efficiency of human PSCs compared with mouse PSCs. Additionally, naïve human PSCs open the gate to study human pre-implantation embryogenesis.In this review, we have written the history, current topics and future direction of PSCs and human naïve PSCs.