著者

大須賀 常良 五十嵐 鉄朗 中村 精二

出版者

一般社団法人日本建築学会

雑誌

大会学術講演梗概集. 計画系

巻号頁・発行日

vol.43, pp.643-644, 1968-09-05

著者

中村 精二 下田 忠次郎

出版者

公益社団法人 日本農芸化学会

雑誌

日本農芸化学会誌 (ISSN:00021407)

巻号頁・発行日

vol.28, no.11, pp.909-913, 1954 (Released:2008-11-21)

参考文献数

10

被引用文献数

3 5

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As reported in the previous paper, one of the authors (SHIMODA) isolated a new antibiotic substance, “oryzacidin”, effective against the “Hiochi” bacteria responsible for the putrefac-tion of Saké from the broth of a strain of Asp. oryzae. But further investigations showed that in the culture medium there were one or two other antibiotic substances effective against “Hiochi” bacteria as well as oryzacidin. We have isolated one of these antibiotics in the crystalline form as its S-benzylthiuronium salt, needles, m.p. 137° (decomp.), from crude Na-oryzacidin powder, and as its free acid, needles, m.p. 67-8°, from the thiuronium salt passing through Amberite I.R. -120. It is soluble in water, alcohol, ethyl acetate, acetone, ether and hot chloroform, fairly soluble in cold chloroform and insoluble in ligroin, petroleum ether and benzene. From the analytical data, the molecular formula of the free acid was found to be C3H5NO4. On the functions of the nitrogen and oxygen, the existences of one nitro and one carboxyl groups were identified from the results of the electrometric titration, Victor MEYER nitrosation test, and infrared absorption spectrum. Reducing with stannous chloride and hydrochloric acid. β-alanine was obtained from its free acid. From these experiments described above, it was concluded that this new antibiotic is identic al with β-nitropropionic acid. Moreover, the synthesized β-nitropropionic acid from β-iodo-propionic acid gave no depression of the melting point when mixed with the substance isolated from broth. The β-nitropropionic acid inhibited the growth of “Hiochi” bacteria at a dilution of 1:30, 000 in the Saké medium. There was found no toxicity in the intravenous injection of 140mg per Kg of mice but mice succumbed within 24 hours to injection of 280mg/kg.

著者

児島 理志 森 晶子 手嶋 尚人 前野 まさる 中村 精二

出版者

一般社団法人日本建築学会

雑誌

学術講演梗概集. F, 都市計画, 建築経済・住宅問題, 建築歴史・意匠 (ISSN:09150161)

巻号頁・発行日

vol.1990, pp.15-16, 1990-09-01