著者

遠藤 英也 久米 文弘

出版者

The Japanese Cancer Association

雑誌

GANN Japanese Journal of Cancer Research (ISSN:0016450X)

巻号頁・発行日

vol.54, no.4, pp.443-453, 1963-12-31 (Released:2008-10-23)

参考文献数

23

被引用文献数

2

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From the fact that 4-hydroxyaminoquinoline 1-oxide (4-HAQO) as well as 4-nitroquinoline 1-oxide (4-NQO) have λ-phage inducing ability in lysogenic Escherichia coli K12 and from the studies of Okabayashi, showing that not only 4-NQO but also 4-HAQO are mutagenic and able to produce the phenotypically same mutants in Aspergillus niger, similarity between these two substances in their biological actions was examined on (1) carcinogenicity, (2) growth-inhibiting action, and (3) intranuclear inclusion-forming action.1) 4-HAQO•HCl in peanut oil and cholesterol mixture was injected into the subcutaneous tissue of rats. Subcutaneous tumors were induced in 8 of the 15 effective rats (53%) in the first group and in 16 of 17 rats (94%) in the second group.2) Three groups, consisting of 10 mice each, were inoculated with Ehrlich ascites tumor cells intraperitoneally. Twenty-four hours after the inoculation, the first group was treated daily with 0.5mg/kg body weight of 4-HAQO•HCl dissolved in physiological saline containing 0.4% carboxymethylcellulose, the second group with 7mg of 4-NQO, and the third with solvent alone. The treatment was continued for 10 days. All the mice of the first group and all but 2 mice of the second survived over 50 days, whereas the third (control) group survived only 15 days.3) When the Chang's liver cells were incubated for 24 hours at 37° in a culture media containing 4-HAQO•HCl in the final concentration of 7×10-5M, intranuclear inclusions phenotypically identical with those induced by 4-NQO were produced in about 60% of all the cells.These results were discussed in relation to the carcinogenic mechanism of 4-NQO.