著者

加藤 幸夫 西村 正宏 菊地 寿幸 澤井 高志

出版者

一般社団法人 日本臨床リウマチ学会

雑誌

臨床リウマチ (ISSN:09148760)

巻号頁・発行日

vol.21, no.1, pp.20-31, 2009-03-30 (Released:2016-03-31)

参考文献数

40

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A rabbit osteoarthritic knee model was used to analyze time-related accessibility of high molecular weight 2,700 kDa hyaluronan to healthy and osteoarthritic articular cartilage and synovium. Fluorescein-labeled high-molecular weight 2,700 kDa hyaluronan (F-HA) was found in shallow, inner and deep layers of degenerated cartilage within 3-24 h after injection. It quickly reached the deep cartilage layer and was retained within inner and deep layers up to day 28. In constrast, in healthy knees, F-HA accessibility was confined to superficial and shallow cartilage layers during 3-24 h and did not change until day 28, showing no signs of deep layer retention. In both healthy and osteoarthritic knees, F-HA penetrated synovium from its synovial lining cell layer to the interstitium in subsynovial fat tissue (deep layer) at 3-24 h after injection. Though having a high molecular weight, 2,700 kDa HA rapidly penetrated shallow, inner and deep layers of degenerated cartilage and deep layer of synovium after its intra-articular injection. The joint tissue penetration of 2,700 kDa HA after its intra-articular injection in an animal OA model indicates that soon after the injection, hyaluronan is rapidly accessible to synovial and degenerated cartilage tissues. There, it exerts its anti-inflammatory actions on the synovium and degeneration-inhibiting action on the articular cartilage.