文献一覧: 塩田勉 (著者)

28 0 0 0 OA 藤枝静男『田紳有楽』-〈イカモノ汎神論〉の地平-

著者: 塩田勉
出版者: 早稲田大学国際教養学部
雑誌: Waseda Global Forum (ISSN:13497766)
巻号頁・発行日: vol.4, pp.83-94, 2008-03

http://hdl.handle.net/2065/29209

5 0 0 0 OA 魚類由来FHM-sp細胞に対する界面活性剤系消火剤の毒性

著者: 森真朗塩田勉
出版者: 日本環境毒性学会
雑誌: 環境毒性学会誌 (ISSN:13440667)
巻号頁・発行日: vol.5, no.2, pp.91-99, 2002 (Released:2010-07-01)
参考文献数: 18

A convenient, simple and rapid cytotoxicity test using cultured cell lines from fish was carried out to know the toxicity of commercial products to fish. Three extinguishing agents (Phos-chek, Jet form III and Miya form) were selected as the commercial products. The FHM-sp cell line, which is the cell line of improved type of the FHM cell line derived from peduncle of fathead minnow, was used as the suspension-cultured cell line from fish. Cytotoxicity of three extinguishing agents to the FHM-sp cell line was determined by neutral red uptake inhibition test. The cytotoxicity of three extinguishing agents was compared to the in vivo toxicity of them obtained by three ecotoxicological tests (algae growth inhibition test, Daphnia magna acute immobilization test and medaka acute toxicity test). The rank order of the in vivo toxicity of three extinguishing agents was the same in three ecotoxicological tests and Phos-chek> Jet form III> Miya form. There is no regulation standard on toxicity of extinguishing agents to aquatic organisms in Japan. Following the regulation standard of National Fire Protection Association (NFPA) in U.S.A., the effect of extinguishing agents tested on aquatic organisms like fish was little in regular use for extinguishing. The rank order of cytotoxicity of them to the FHM-sp cells was Phos-chek> Miya form > Jet form III. However, the difference of cytotoxicity value between Miya form and Jet form III was a little. The value of 24h NR50 to FHM-sp cells was near to that of 96h LC50 to medaka. Therefore the sensitivity of the in vitro toxicity test using the FHM-sp cells to three extinguishing agents was similar to that of the in vivo toxicity test using medaka. The toxicity values of three ecotoxicological tests (72hEC50, 48hEC50, 96hLC50) and the cytotoxicity test (24hNR50) to three extinguishing agents were in the same range and 10-50 mg/L. This shows that the in vitro toxicity test using the FHM-sp cells is useful to estimate the range of test concentration for in vivo toxicity tests.