著者

小合 宗一

出版者

口腔病学会

雑誌

口腔病学会雑誌 (ISSN:03009149)

巻号頁・発行日

vol.54, no.1, pp.37-46, 1987

被引用文献数

1

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In order to elucidate cell surface molecules involved in the craniofacial morphogenesis, we adopted the development of the lens placode as a model system. We raised a rabbit antibody (anti-C1) which inhibits Ca<SUP>2+</SUP>dependent cell-cell adhesion of Cl cell, a cell line derived from the fetal rat lens epithelial tissue, and examined the effects of anti-C1 on the lens development of the rat embryos under the whole embry culture. <BR>The rat embryos were collected from the uteri at day 11. The anti-C1 Fab fragments dissolved in Hanks saline were injected near the eye anlagen with a glass capillary of about 30 pm in diameter. The injected embryos were incubated with the whole embryo culture system for 20 hours. The preimmune rabbit Fab fragments and Hanks saline alone were also injected as controls. After incubation, most of the embryos injected with the anti-C1 Fab showed an abnormal lens development, in particular, the incomplete separation of lens vesicle from epidermis, while embryos injected with Hanks saline or control antibodies showed a complete segregation of lens vesicle. <BR>It is suggested that the Ca<SUP>2+</SUP>-dependent cell adhesion molecule of Cl cell is the target molecule of anti-C1 which is involved in the lens development. The identification of the target molecule is in progress by raising monospecific antibodies.