著者

山垣 亮 木村 優佳 山崎 堯嗣

出版者

一般社団法人 日本質量分析学会

雑誌

Journal of the Mass Spectrometry Society of Japan (ISSN:13408097)

巻号頁・発行日

vol.69, no.2, pp.23-29, 2021-04-01 (Released:2021-04-15)

参考文献数

8

被引用文献数

1

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We optimized the suppression method of peptide adsorption of neuropeptide Y (NPY) in the experimental tubes for the sensitive quantitative LC-MS analysis. A large peptide of Orexin-B and small tryptic digested bovine albumin (BSA) peptides suppressed effectively the adsorption of NPY in 35–50% acetonitrile with 0.1% trifluoroacetic acid aqueous solution. Orexin-B adsorbed on the experimental tubes competitively to NPY, and the tryptic digested BSA peptides suppressed the aggregation of NPY inducing the adsorption or the deposition of NPY on the tube in the solution.