著者
吉野 健一
出版者
一般社団法人 日本質量分析学会
雑誌
Journal of the Mass Spectrometry Society of Japan (ISSN:13408097)
巻号頁・発行日
vol.55, no.2, pp.99-115, 2007 (Released:2007-04-15)
参考文献数
27
被引用文献数
6 6

Ions are not organisms, and thus they do not proliferate by either sexual or asexual reproduction. They are hence incapable of being parents, children, or grandchildren. Therefore, the terms “parent ion,” “daughter ion,” and “granddaughter ion” are scientifically incorrect. In addition, mass spectrometrists should note that the terms “daughter ion” and “granddaughter ion” are gender-specific. (The terms “daughter nuclide” and “granddaughter nuclide” are also gender-specific.) Therefore, the terms “parent ion” and “daughter ion” need to be replaced by “precursor ion” and “product ion,” respectively. Instead of the gender-specific terms “granddaughter ion” and “great-granddaughter ion,” the gender-neutral terms “second generation product ion” and “third generation product ion” should be used.
著者
山垣 亮 木村 優佳 山崎 堯嗣
出版者
一般社団法人 日本質量分析学会
雑誌
Journal of the Mass Spectrometry Society of Japan (ISSN:13408097)
巻号頁・発行日
vol.69, no.2, pp.23-29, 2021-04-01 (Released:2021-04-15)
参考文献数
8

We optimized the suppression method of peptide adsorption of neuropeptide Y (NPY) in the experimental tubes for the sensitive quantitative LC-MS analysis. A large peptide of Orexin-B and small tryptic digested bovine albumin (BSA) peptides suppressed effectively the adsorption of NPY in 35–50% acetonitrile with 0.1% trifluoroacetic acid aqueous solution. Orexin-B adsorbed on the experimental tubes competitively to NPY, and the tryptic digested BSA peptides suppressed the aggregation of NPY inducing the adsorption or the deposition of NPY on the tube in the solution.
著者
吉野 健一
出版者
一般社団法人 日本質量分析学会
雑誌
Journal of the Mass Spectrometry Society of Japan (ISSN:13408097)
巻号頁・発行日
vol.56, no.1, pp.27-32, 2008-02-01 (Released:2008-02-26)
参考文献数
2
被引用文献数
1 1

The terms “accurate mass” and “exact mass” are not synonymous in mass spectrometry. Accurate mass refers to the mass value of an ion or radical determined experimentally by mass spectrometry. On the other hand, exact mass refers to the mass value of a single ion, radical, or molecule calculated by summing the mass values of individual isotopes. To avoid confusion, “measured accurate mass” and “calculated exact mass” should be used in place of “accurate mass” and “exact mass,” respectively.
著者
吉野 健一
出版者
一般社団法人 日本質量分析学会
雑誌
Journal of the Mass Spectrometry Society of Japan (ISSN:13408097)
巻号頁・発行日
vol.55, no.6, pp.381-388, 2007 (Released:2007-12-15)
参考文献数
9
被引用文献数
1 1

In terms related to mass spectrometry, “TIC” should be used as an abbreviation for “total ion current.” However, many mass spectrometrists use “TIC” as an abbreviation for “total ion chromatogram” while referring to “total ion current chromatogram,” which is incorrect. Another common misunderstanding related to abbreviations is the usage of “MCP” as an abbreviation for “multichannel plate,” which should be used for “microchannel plate.”
著者
檜山 英三 原田 隆範 兒島 正人
出版者
一般社団法人 日本質量分析学会
雑誌
Journal of the Mass Spectrometry Society of Japan (ISSN:13408097)
巻号頁・発行日
vol.68, no.2, pp.33-37, 2020-04-01 (Released:2020-04-15)
参考文献数
2

Circulating tumor cell (CTC) has improved the study of cancer disease as it represents a noninvasive biopsy that can be used as prognostic and prediction biomarkers as well as for future molecular diagnosis as an accurate selection of molecular targets and effective drugs. As tumor heterogeneity exists in neuroblastoma (NBL), we aimed to evaluate lipid-metabolomic molecular profile as well as genetic profiles at the single cell level of CTC for better understanding the heterogeneity and malignant grade of NBL. For attempt of CTC analysis in NBL patients, we have isolated single-CTCs and then performed direct trapping of a single cell within a nanospray tip followed by super-sonication after the addition of ionization solvent for metabolomics analysis. And we also performed genomic aberrations and expressions using next-generation sequencing after whole genome/transcriptome amplifications (WGA/WTA). In the NBL-CTCs, metabolomic analysis detected catechol amine metabolites, which are specific to NBL, and drugs included in the patient’s course of therapy in addition to vital molecules such as amino acids. In genomic analysis, the average amount of amplified DNA was 19.0 µg, and the percentage of reads mapped to any targeted region relative to all reads mapped to the reference was 66.5–98.9%. The concordance rates of CTC and tumor mutations were 49–80%. The genomic expression analysis in CTC showed the activation of cancer metastatic, neurogenic, stem cell signaling pathway genes. This “direct single-cell analysis method” seems to be useful for direct and wide range genomic and molecular marker detection in NBL-CTC, for evaluating heterogeneity of NBL tumor at diagnosis as well as during treatments. This method will be applicable for selection of effective drugs and evaluation of treatment efficacy in future.
著者
角田 紀子
出版者
一般社団法人 日本質量分析学会
雑誌
Journal of the Mass Spectrometry Society of Japan (ISSN:13408097)
巻号頁・発行日
vol.53, no.3, pp.157-163, 2005 (Released:2006-04-05)
参考文献数
38
被引用文献数
1 2

2004 was the 10th anniversary of the Matsumoto sarin incident, and 2005 is the 10th anniversary of the Tokyo Subway Attack. National Research Institute of Police Science has been engaged in forensic examinations into these incidents. Chemical analyses of the victim's blood, water, soil, and wipe samples were performed by organic solvent extraction, followed by gas chromatography-mass spectrometry with or without tert-butyldimethylsilylation. As a result, sarin and its hydrolysis products (isopropylmethyl phosphonate and methylphosphonate), derived from sarin were positively identified by their mass spectra and retention indices. Furthermore, from the chemical analysis of evidence samples taken from the scene of manufacturing plant, precusors, and byproducts corresponding to synthetic routs of sarin has been identified. This paper presents characteristics of nerve agents, sample preparation of sarin, optimization of GC-MS, and case reports from a standpoint of effectiveness of GC-MS.
著者
吉野 健一
出版者
The Mass Spectrometry Society of Japan
雑誌
質量分析 = Mass spectroscopy (ISSN:13408097)
巻号頁・発行日
vol.56, no.2, pp.69-76, 2008-04-01
参考文献数
7
被引用文献数
1

The Japanese word "mirimasu" originates from the English words "milli" and "mass." It is normally used as an alias for "measured accurate mass" and "accurate mass measurement." It causes confusions that "mirimasu" is also used as an alias for "high resolution mass spectrometry," "high resolution mass spectrum (spectra)," and "mass spectrometer with high mass resolving power." This nomenclature results from the fact that "high resolution" is confused with "high accuracy." In addition to these interpretations, Japanese mass spectrometrists also use "mirimasu" as an alias for "one-thousandth of a unified atomic mass unit," "one-thousandth of a dalton (mDa)," and "one-thousandth of an <i>m/z</i> unit." This incorrect usage is due to a lack of understanding of the unit system of atomic mass and <i>m/z</i> on mass spectra. Therefore, "mirimasu" should not be used in Japanese, because it is ambiguous and involves various problems and misunderstandings.