著者

山科 孝雄 平田 博文

出版者

Japan Oil Chemists' Society

雑誌

油化学 (ISSN:18842003)

巻号頁・発行日

vol.36, no.6, pp.441-443, 1987-06-20 (Released:2009-11-10)

参考文献数

4

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The reactions of amino groups in amino acids and proteins with sodium 2, 4, 6-trinitrobenzenesulfonate (TNBS·Na) in the presence of sodium sulfite (Na2SO3) were studied at pH 10.1. Determination of the amino groups was performed by following the absorbance A (420 nm) of the Meisenheimer complex. A good linear relationship between A and the concentrations of amino compounds was observed. The reactions in a cuvette showed good agreement with those in the dark. Based on these observations, a modified method for determining amino groups by TNBS·Na in the presence of Na2SO3 is proposed, and several comments are described.