著者

藤原 京子

出版者

日本ウイルス学会

雑誌

ウイルス (ISSN:00426857)

巻号頁・発行日

vol.9, no.6, pp.550-558, 1959-12-20 (Released:2010-03-16)

参考文献数

13

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The mode of invasion of virus from a traumatized area to the brain and to the salivary gland is the most important problem in the pathogenesis of rabies. In order to obtain a clue to solve this problem, suckling mice were used as experimental animals and following results were obtained.1) Adult and suckling mice were inoculated with fixed virus of rabies by various routes, and their susceptibilities were compared. In intracerebral inoculation, the susceptibility of suckling mice was slightly, but significantly higher than that of adult mice. When inoculated subcutaneously, the difference was more evidently observed, suckling mice being about 100 times as susceptible as adult ones. However, the susceptibility of suckling mice inoculated subcutaneously was far below that of adult mice inoculated intracerebrally.2) The virus neutralizing activity of immune serum was tested by two ways, i.e. intracerebral inoculation into adult mice and subcutaneous inoculation into suckling mice. The results showed that the sensitivity of neutralization test could not be enhanced by suckling mice technique.3) The distribution of virus in organs of infected mice was examined. A small amount of virus was always found in the salivary gland of suckling mice, although the same organ of adult mice showed no virus activity.4) Serial passages of brain and salivary gland viruses in suckling mice were carried out by intracerebral or subcutaneous route of inoculation. The four strains thus obtained were examined for their pathogenecity for mice, such as the ability to infect after peripheral inoculation or to appear in salivary gland tissue. But these four strains were not different in any respects from original one.From these results, some points concerning the process of invasion of rabies virus into central nervous system of infected animals were discussed.