著者

加来 博 小島 晃 林 孝次 堀井 正清 中村 清文 藤田 〓也

出版者

International Society of Histology and Cytology

雑誌

Archivum histologicum japonicum (ISSN:00040681)

巻号頁・発行日

vol.23, no.1, pp.7-19, 1962-10-20 (Released:2009-02-19)

参考文献数

8

被引用文献数

4 2

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The authors studied the kinetics of the intestinal epithelium of mouse, and discussed the problems of cellular proliferation and differentiation.The results obtained are as follows:1. By colchicine method the generation time and mitotic duration of the crypt cell are estimated from the increase in counts of mitotic figures.2. By 3H-thymidine flash labeling autoradiography the authors divided mucosal lining of the crypt into two zones: ‘generation zone’ and ‘PANETH cell zone’. The former comforms to DNA-synthesizing zone and the latter consists of the PANETH cells.3. By pursuit of the label in the generation zone, it is proved that the villus cells go up to top of the villus and fall off into the lumen. Accordingly the life span of the villus cell is estimated.4. The generation time (tG), presynthetic time (tI), synthetic time (tS), postsynthetic time (t2) and mitotic duration (tM) are analysed by means of 3H-thvmidine autoradiography.5. The PANETH cells in the bottom of the crypt fall into the lumen of the crypt discharging their secretory granules.6. The intestinal epithelium consists of the villus cells, the PANETH cells and the generative cells. Only the generative cells are proliferating and supply cells to the other zones.7. These three kinds of epithelial cells constitute three compartments respectively with a settled bulk. The three compartments are bound in a controled balance.8. The cytodifferentiation from the generative cells into the villus cells and into the PANETH cells seems to be determined within a transitional zone, i. e., ‘differentiation zone’. The cells which enter in the differentiation zone during their presynthetic time are determined to differentiate into villus cells or PANETH cells and lose their capacity of DNA synthesis.9. It is infered that at the point of maturation from the generative cell into the villus cell or into the PANETH cell, environmental factors in differentiation zone give decisive influence on the cellular differentiation.10. The 3H-thymidine autoradiographic study on the stomach epithelium of Hamster reveals that the stomach epithelium also has ‘Generation zone’ in transitional portion between the surface epithelium and the glandular cells. The glandular cells in the stomach may be seen as homologous to the PANETH cells in intestinal epithelium from the standpoint of cytokinetics.