著者

Daisuke Koga Tatsuo Ushiki

出版者

International Society of Histology and Cytology

雑誌

Archives of Histology and Cytology (ISSN:09149465)

巻号頁・発行日

vol.69, no.5, pp.357-374, 2006 (Released:2007-03-12)

参考文献数

39

被引用文献数

20 34

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The three-dimensional ultrastructure of the Golgi apparatus in different cells of the rat - epithelial principal cells in the epididymal duct, goblet cells in the jejunum, gonadotrophs in the pituitary gland and dorsal root ganglion cells - was studied by scanning electron microscopy (SEM) of osmium-macerated tissues. The Golgi apparatus in the epididymal principal cells took the shape of a candle flame with irregular-shaped cisterns, while those in the goblet cells of the jejunum were cup-shaped or cylindrical with flat cisterns. Gonadotrophs had a large spherical Golgi apparatus; this apparatus was composed of several concentric cisterns with large round windows through which the rough endoplasmic reticulum (rER) and mitochondria extended into the center of the globular Golgi apparatus. Dorsal root ganglion cells had several small Golgi stacks scattered in the cytoplasm. In all Golgi apparatuses of the different cells examined in the present study, the cis-most cistern was generally composed of a flattened sheet with numerous small fenestrations on its wall. On the other hand, the shape of the trans-most cistern varied by cell type; it was generally composed of tubules and/or small sheets which were sometimes connected with each other to form a rather complicated structure. The cis-most cistern and the trans-most cistern were often closely associated with the rER although no direct communication was found between them. These findings indicate that the structure of the Golgi apparatus, especially its overall shape and the ultrastructure of the trans-most cistern, varies by cell type, a point to be considered in relation to the function of the individual cells.

著者

小林 寛 Masahiko KUMAKURA Ken YOSHIMURA Mititomo INATOMI Tomoichiro ASAMI

出版者

International Society of Histology and Cytology

雑誌

Archives of Histology and Cytology (ISSN:09149465)

巻号頁・発行日

vol.61, no.1, pp.37-46, 1998 (Released:2011-10-26)

参考文献数

28

被引用文献数

53 57

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The tongue in four species of penguin was investigated by light microscopy and by scanning electron microscopy, with special reference to the lingual papillae.1) The middle of the penguin tongue contains a pair of long cartilages and long tendons accompanied by striated muscle bundles.2) Large, spine-like, and caudally directed lingual papillae (filiform-like papillae) densely cover the dorsal surface of the tongue, apparently serving to catch fishes.3) By light microscopy, the dorsal lingual epithelium with the lingual papillae are seen as a thick cornified layer, but the lateral and lower surfaces have a thinner cornified layer.4) The connective tissue core (CTC) under the epithelium of the lingual papilla shows a stereo structure similar to but smaller than the external form of the papilla. The CTC contains some blood vessels and nerve fibers; the lingual glands are found in the submucous layer only in the posterior tongue.5) Bundles of nerve fibers in the lamina propria of the tongue were immunohistochemically positive for PGP 9.5, and it appeared that Merkel corpuscles were distributed in the connective tissue closely beneath the epithelium of the finger-like papillae, though they were only weakly immunoreactive for PGP 9.5.6) Numerous fine filaments of elastic fibers are found closely beneath the epithelial cell layer of the dorsal surface of the tongue including the lingual papillae, while there are very few of them on the lateral and lower sides of the tongue.

著者

山本 正夫 Katsuko KATAOKA

出版者

International Society of Histology and Cytology

雑誌

Archivum histologicum japonicum (ISSN:00040681)

巻号頁・発行日

vol.49, no.4, pp.449-457, 1986 (Released:2011-10-26)

参考文献数

28

被引用文献数

20 39

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A primary cilium often projected from the Golgi region of pancreatic A, B and D cells. The proximal portion of the cilium was found surrounded by a tubular invagination of the plasmalemma, and then the cilium extended into the intercellular canaliculus. The most proximal portion of the ciliary membrane exhibited periodical densities which might correspond to the ciliary necklace. The axoneme of the cilium was basically of the 9+0 pattern, i. e., nine peripheral doublets and no central singlet, though it was modified along the length of the cilium. Although a few appendages were projected from each doublet, it was difficult to identify dynein arms and nexin links. At the most proximal portion of the cilium, a “champagne-glass” structure connected each doublet with the ciliary membrane.The distal and proximal centrioles of the diplosome were connected to each other by a striated band. The proximal centriole, which served as a basal body, had accessory structures, such as alar sheets, basal feet and rootlets.Frequent projections of the primary cilium and its elaborate structure suggest that the cilium is not an aberrant structure but rather one which plays a certain role in the islet cell function.

著者

養老 孟司 神谷 敏郎

出版者

International Society of Histology and Cytology

雑誌

Archivum histologicum japonicum (ISSN:00040681)

巻号頁・発行日

vol.35, no.5, pp.403-415, 1973

被引用文献数

4

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北海道産オオアシトガリネズミの放臭腺の構造と, この腺の大部分を占めるアポクリン腺の腺細胞の微細構造を記載した.<br>放臭腺は毛とこれに付属する脂腺, アポクリン腺が側胸部でとくに良く発達したもので, 雄では大きく, 雌では小さい.<br>アポクリン腺の腺細胞は分泌物の量により異った形を示す. 分泌物は蛋白を多く含むと思われるが, 限界膜をもたず, 周囲にはリボソームが集る. ミトコンドリアの形態と分布は特異で, 細胞の基底部では多形性を示し, 小さく, 分泌物との関連がはっきりしないが, 頂部では丸く大きく, 基質電子密度は高く, 分泌物の周囲をとりかこむように配列する. この種の大きなミトコンドリアは分泌物とともに細胞外へ出される可能性がある.<br><i>Sorex</i> 属では放臭腺は主として腺体がアポクリン腺で占められているが, 近縁の <i>Neomys</i> 属と <i>Crocidura</i> 属では脂腺で占められる. これとちょうど平行した属間変更を示すのが顎下腺であって, 線条部が <i>Sorex</i> では分泌能がなく, <i>Neomys, Crocidura</i> では分泌能をもつ. このことから想定される顎下腺線条部と放臭腺の機能的関連について論じた.

著者

Shohei YAMASHINA Hideaki TAMAKI Osamu KATSUMATA

出版者

International Society of Histology and Cytology

雑誌

Archives of Histology and Cytology (ISSN:09149465)

巻号頁・発行日

vol.62, no.4, pp.347-354, 1999 (Released:2005-12-01)

参考文献数

26

被引用文献数

9 17

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The ultrastructure of the secretory endpiece of the rat sublingual gland was examined in samples prepared by rapid freezing and freeze-substitution method, and results were analyzed in combination with 3-D images reconstructed by computer graphics from light micrographs of serial sections. Fixation by rapid freezing followed by freeze-substitution preserved cellular ultrastructures, especially the membrane structure, in perfect condition, and demonstrated the terminal portion of the sublingual gland to be a compound branched tubulo-alveolar gland with serous cells distributed throughout the end-pieces. All the serous cells aligned with mucous cells to surround a common lumen, leaving no demilune structure. In contrast, samples fixed by the conventional immersion method showed distended mucous cells displacing the serous cells toward the basal portion of the acinus to form the demilune structure. The luminal space was also compressed and appeared disconnected from the serous cells. From these observations, the serous demilune that for more than 130 years has been believed to be an actual histological entity was proved to be an artificial structure produced through compression by the hydrated and expanded mucous cells during immersion fixation.

著者

鈴木 安恒 長田 光博 渡辺 昭

出版者

International Society of Histology and Cytology

雑誌

Archivum histologicum japonicum (ISSN:00040681)

巻号頁・発行日

vol.23, no.5, pp.431-446, 1963

被引用文献数

5

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In addition to lung and gill respirations, there is a specific type of respiration in the <i>Misgurnus anguillicaudatus</i> which is generally called 'Loach's intestinal respiration' and is considered to be of as large as one third of the total respiration volume.<br>Many experimental and physiological studies about the intestinal respiration have been made (SUYEHIRO 1933, KOYAMA 1958 etc.), yet no one has made any morphological study up to the present.<br>After our cytological and electronmicroscopical studies of matured loach's intestines it has been confirmed that the respiration is carried out by straight intestine (lower intestine) and especially that columnar cells each are to play an active part in it. Furthermore comparative studies have been made between lung epithelium (LOW 1952, BARGMANN and KNOOP 1956, KARRER 1956 and GRONIOWSKI and DJACZENKO 1961) as well as gill epithelium (HAYEK 1960, MIZUHIRA et al. 1962) and loach's lower intestine epithelium from the respiratory functional view point.<br>The results obtained are summarized as follows;<br>Material and methods: Matured loachs (<i>Misgurnus anguillicaudatus</i>) were sacrificed after one day's starvation, and upper intestine (coiled intestine) and lower intestine (straight intestine) were extracted and fixed in LEVI'S solution, ZENKER-formol and 10% formalin. For the electron microscopy, small pieces of both intestines were fixed in 1% osmium tetroxide adjusted to pH7.4 with phosphate buffer, dehydrated through a series of alcohol, and then embedded in styrene and n-butyl methacryrate (1:1), epon, and new epoxy resine 'epok 533' recommended by KUSHIDA's method. Thin sections were cut on a PORTER-BLUM microtome with glass knives, stained with uranyl acetate, and examined in a HITACHI's HU-10 electron microscope and a NIPPON DENSHI's JEMT-6 electron microscope at magnification of 1, 000-10, 000.<br>1. The epithelium of the intestinal mucous membrane is composed of one layer of columnar cells. Villi are well developped in the upper intestine where columnar cells form a great majority of the intestinal epithelial cells, and goblet cells are recognized scatteringly. These columnar cells take slender columnar form and have striated border. The nuclei are round or oval in shape and are found at the basal part of the cell. Mitochondoria are of a short thread shape or a short bacilli-form and mostly gather at the apical portion of the cell. Small arteries and capillaries are observed in the lamina propria of the mucous membrane.<br>2. In the lower intestine (straight intestine), no villi are observed and yet goblet cells are found in comparatively great numbers. What especially differs from the upper intestine (coiled intestine) is the fact that blood capillaries get into intercellular spaces of the columnar cells from the lamina propria, and widen their space and swell toward the intestinal lumen. As a result the surrounding columnar cells remarkablly transform themselves because of the pressure of the capillaries, turn into a thin layer and lose their striated border.<br>These phenomena are recognized in almost all parts of the lower intestine and are particularly remarkable about the anus.<br>However, as far as goblet cells are concerned, their form differs according to the mass of the mucous contents as in the upper intestine, and the above said insertion of the capillaries has practically no deforming effect upon their form. Furthermore, they open into the intestinal lumen with a small stoma, and do not cover the intestinal side of capillaries.<br>Consequently it is the columnar cells that perform the respiration, while goblet cells only assume charge of producing mucus for the smooth discharge of feces through the intestinal cavity.<br>No PANETH's cells and no chromaffin cells are found.<br>The intestinal muscle layers consist of inner circular and outer longitudinal layers

著者

Rufus M. CLARKE 小林 繁

出版者

International Society of Histology and Cytology

雑誌

Archivum histologicum japonicum (ISSN:00040681)

巻号頁・発行日

vol.38, no.2, pp.133-150, 1975 (Released:2009-02-20)

参考文献数

11

被引用文献数

4 7

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ラットの空腸を分離 切断し, その先端は縫合され, 後端は腹壁に開口する袋を手術的に作製した. 次いで この袋の先端近くより, 量と成分の知られている種々の試験液を注入し, 粘膜の細胞学的変化を光線顕微鏡と電子顕微鏡を使って経時的に研究した.ポリエチレングリコール4000 (PEG) の等張液 (16.8%) を注入したものでは, 腸絨毛の上端部の腸細胞はすみやかに損傷された. しかし杯細胞と基底果粒細胞には変化が認められなかった. 等張PEG注入後6-72時間後に採取された標本では, 腸絨毛の上端に杯細胞と基底果粒細胞よりなる帽子状の細胞塊が形成されていた. 1% PEGと5.1%ブドウ糖の混合液を注入したものでは, 注入液が直接注ぐ袋の先端部の粘膜には変化がみられないが, 袋の中間部と後部では, 等張PEGを注入したときと同様の著明な変化が認められた. 蒸溜水の注入も, 腸細胞を損傷したが, このさいの粘膜の変化はPEG注入によるものとは 明らかに異っていた. 等張ブドウ糖液の注入では 粘膜の形態はまったく正常であった. PEG注入による腸粘膜の損傷は, PEG注入を止めてのち, 等張ブドウ糖液を6時間注入することによって正常に回復した.以上の結果が, 腸絨毛を被う上皮細胞の絶えざる更新の問題との関連において考察された.

著者

Mituhiko HISADA

出版者

International Society of Histology and Cytology

雑誌

Archives of Histology and Cytology (ISSN:09149465)

巻号頁・発行日

vol.52, no.Supplement, pp.139-146, 1989 (Released:2011-10-26)

参考文献数

26

被引用文献数

2 2

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We have found a large portion of the neurons in the abdominal ganglia of the crayfish, Procambarus clarkii (Girard) to be local nonspiking neurons. These neurons are anaxonic, have extensive arborizations of neurites and have their entire structure confined within a single ganglion. They show relatively low membrane potentials of 40-50mV and under no circumstance generate action potentials, yet their membrane potential changes can effectively modulate the activity of postsynaptic neurons which are generally motoneurons. The transmission is chemical and transmitter release in many, if not all, of them is continuous even at their “resting” level. Electron microscope examination showed an extensive intermingled distribution of both input and output synaptic structures. This synaptic distribution together with the passive electric property suggests that individual neurites of this type of neurons function rather independently, constituting numerous local circuits. Similar nonspiking communication is also found among the ordinary “spiking” motoneurons, indicating that this mode of communication is far more widely used in the crayfish central nervous system than generally believed. These findings appear to necessitate a radical revision of our understanding of how the central nervous system operates in arthropods.

著者

村上 弦 Tatsuo SATO Tohru TAKIGUHI

出版者

International Society of Histology and Cytology

雑誌

Archives of Histology and Cytology (ISSN:09149465)

巻号頁・発行日

vol.53, no.Supplement, pp.219-235, 1990 (Released:2011-10-26)

参考文献数

14

被引用文献数

18 22

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This article aims to clarify the topographical relationships of the bronchomediastinal collecting lymph vessels to other structures, in particular the great vessels, the trachea, the esophagus and the mediastinal pleura. Minute dissection was performed on eight cadavers with special reference to the converging collecting lymph vessels which form the bronchomediastinal trunks.On the right side, the trunks were consistently observed on both the right brachiocephalic vein and the subserous surface of the mediastinal pleura (anterior and posterior mediastinal trunks). The pathway from the right recurrent chain nodes ran laterally behind the carotid sheath and led either into the deep cervical nodes situated on the scalenus anterior or directly into the right venous angle.On the left side, the trunks showed varying courses. The nodes from which the trunks arose were constant, and classifiable into three groups: the uppermost paratracheal nodes near the recurrent chain nodes, the anterior mediastinal nodes (the left phrenic nodes) surrounding the phrenic nerve in front of and inferior to the aortic arch (the origin of the superior mediastinal trunk), and the left tracheobronchial nodes (the origin of the inferior mediastinal trunk).The large transverse superficial communicating vessel between the right and left sides was usually found in front of the trachea above the aortic arch; it was often connected to the nodes of the brachiocephalic angle. Deep communications were also found in front of the carina and behind the trachea.These findings allow the collecting vessels from the thoracic viscera to be divided into two pathways on each side: the anterior and posterior mediastinal trunks on the right side, and the superior and inferior mediastinal trunks on the left side. In addition to the four trunks, the superficial communicating vessel between the right and left sides is also drained from the superior mediastinum. The internal mammary lymph chain, which often emptied directly into the venous angle or into the deep cervical nodes, occasionally joined with the right anterior mediastinal trunk or the left superior mediastinal trunk.

著者

平林 朗

出版者

International Society of Histology and Cytology

雑誌

Archivum histologicum japonicum (ISSN:00040681)

巻号頁・発行日

vol.5, no.1, pp.63-69, 1953-04-20 (Released:2009-02-19)

参考文献数

9

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Nach einer Reihe von Vorversuchen an Meerschweinchen wurden an der formalinfixierten Leber aus Leichen des Menschen, deren Todesursache und Todeszeit bekannt waren, die postmortalen Veränderungen der Erythrocyten untersucht, und zwar in der Zeit vom Sommer bis zum Winter. Dabei wurde das Leberstück in Celloidin eingebettet und in Schnitte zerlegt, welche mit Phosphormolybdän-Kernechtrot und Trypanblau gefärbt wurden. Die Erythrocyten vermindern im Laufe der Zeit die Färbbarkeit mit Kernechtrot, vermehren aber die Färbbarkeit mit Trypanblau und quellen auf, um schließlich nach 96 Stunden gänzlich zu zerfallen. Die Ultrastrukturdichte der Zellen erniedrigt sich also nach dem Tode. Auf Grund dieser Veränderungen ist umgekehrt die Todeszeitbestimmung möglich. Die Tafelabbildungen zum Artikel, Skizzen von je 50 Erythrocyten in der Zeit 10, 20, 30, 48 und 72 Stunden nach dem Tode, leisten Dienste in der gerichtlich-medizinischen Praxis.

著者

加来 博 小島 晃 林 孝次 堀井 正清 中村 清文 藤田 〓也

出版者

International Society of Histology and Cytology

雑誌

Archivum histologicum japonicum (ISSN:00040681)

巻号頁・発行日

vol.23, no.1, pp.7-19, 1962-10-20 (Released:2009-02-19)

参考文献数

8

被引用文献数

4 2

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The authors studied the kinetics of the intestinal epithelium of mouse, and discussed the problems of cellular proliferation and differentiation.The results obtained are as follows:1. By colchicine method the generation time and mitotic duration of the crypt cell are estimated from the increase in counts of mitotic figures.2. By 3H-thymidine flash labeling autoradiography the authors divided mucosal lining of the crypt into two zones: ‘generation zone’ and ‘PANETH cell zone’. The former comforms to DNA-synthesizing zone and the latter consists of the PANETH cells.3. By pursuit of the label in the generation zone, it is proved that the villus cells go up to top of the villus and fall off into the lumen. Accordingly the life span of the villus cell is estimated.4. The generation time (tG), presynthetic time (tI), synthetic time (tS), postsynthetic time (t2) and mitotic duration (tM) are analysed by means of 3H-thvmidine autoradiography.5. The PANETH cells in the bottom of the crypt fall into the lumen of the crypt discharging their secretory granules.6. The intestinal epithelium consists of the villus cells, the PANETH cells and the generative cells. Only the generative cells are proliferating and supply cells to the other zones.7. These three kinds of epithelial cells constitute three compartments respectively with a settled bulk. The three compartments are bound in a controled balance.8. The cytodifferentiation from the generative cells into the villus cells and into the PANETH cells seems to be determined within a transitional zone, i. e., ‘differentiation zone’. The cells which enter in the differentiation zone during their presynthetic time are determined to differentiate into villus cells or PANETH cells and lose their capacity of DNA synthesis.9. It is infered that at the point of maturation from the generative cell into the villus cell or into the PANETH cell, environmental factors in differentiation zone give decisive influence on the cellular differentiation.10. The 3H-thymidine autoradiographic study on the stomach epithelium of Hamster reveals that the stomach epithelium also has ‘Generation zone’ in transitional portion between the surface epithelium and the glandular cells. The glandular cells in the stomach may be seen as homologous to the PANETH cells in intestinal epithelium from the standpoint of cytokinetics.

著者

吉江 紀夫 本間 義治

出版者

International Society of Histology and Cytology

雑誌

Archivum histologicum japonicum (ISSN:00040681)

巻号頁・発行日

vol.38, no.5, pp.339-346, 1976 (Released:2009-02-20)

参考文献数

7

被引用文献数

1 2

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熱帯産の雌ヒメウミガメを用い, 特異的に発達した食道を光顕ならびに走査電顕により観察した. 食道は, 著しく角化した重層扁平上皮よりなり, その大部分は多数の突起 (別名 咽頭歯) となっている. 食道上皮は3層すなわち胚芽層, 有棘層 および角質層からなる. 突起の表面は, 鱗片状に剥離していく角化細胞によっておおわれており, 角質層が極めて厚く, 100μに達する. これは, 他の食道粘膜上皮角質層の3∼7倍の厚さである.顎歯を欠くウミガメにみられた これら特異な形状の突起は, 飲みこんだ食物を 細片にくだくのに役立っているらしい.

著者

Manfred UECK 和気 健二郎

出版者

International Society of Histology and Cytology

雑誌

Archivum histologicum japonicum (ISSN:00040681)

巻号頁・発行日

vol.40, no.Supplement, pp.261-278, 1977 (Released:2011-10-26)

参考文献数

63

被引用文献数

8 18

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松果体は間脳の第三脳室上壁が管状に膨出して発生した器官で, その主たる構成細胞である松果体細胞 (pinealocyte) はニューロンではないが, 神経性上皮に由来する.松果体細胞は, 下等脊椎動物では, 網膜の視細胞と同様な外節を持ち, 光を直接受容する. 外節構造は系統発生の過程において次第に退化し, 哺乳類ではもはや存在しない. 光受容性の松果体細胞は僅かに内分泌機能を保有するが, は虫類から鳥類, 哺乳類へと移行するに従い, 内分泌機能は増強し, ゴルジ装置由来の分泌果粒が毛細血管に相対する細胞突起の終末に蓄積している. 光受容性の松果体細胞は松果体に分布する知覚性ニューロンとの間にリボンシナプスを形成する. 知覚性ニューロンは哺乳類松果体にはもはや存在しない. 一方 自律神経線維は光受容性松果体にはわずかに認められるに過ぎないが, 鳥類, 哺乳類には密に分布している. 環境の光の影響下にある交感神経支配によって松果体細胞のホルモン合成に関与する酵素の活性が調節される. メラトニンのようなインドール誘導体の合成が証明され, ペプチドホルモンの存在が議論されている.以上のように, 松果体は神経-化学, 神経-内分泌変換器, あるいは光入力 (下等脊椎動物においては直接的に, 哺乳類においては交感神経を介して) をホルモン出力に変換する光-神経-内分泌器官として機能する. 松果体は光環境の歯車と噛み合った“生物時計”の役割を演じ, 生体のいくつかの機能の調節器 (または調節器の調節器) でもある. 哺乳類では生殖機能との相関がよく知られている. 松果体細胞は脳脊髄液接触ニューロンと神経分泌細胞とのそれぞれの特徴を兼ね備えている. また藤田恒夫 (1976) の定義に従えばパラニューロンの一つであるとも云える.

著者

小林 繁

出版者

International Society of Histology and Cytology

雑誌

Archivum histologicum japonicum (ISSN:00040681)

巻号頁・発行日

vol.31, no.5, pp.511-528, 1970 (Released:2009-02-20)

参考文献数

38

被引用文献数

9 6

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シマヘビ65ヒキの種々の器官を電子顕微鏡下に観察して, 約3割の個体の血中に従来記載のない特異なコロイド粒子を認めた. この粒子は高電子密度球型の小体で, 直径約20mμあり, 血液中と血管周囲の組織腔に分布していた.毛細血管壁ではこの粒子は内皮細胞の膜構造物, 内皮下腔, 基底膜中にみられ, これらの所見はこの粒子の毛細血管壁通過を示すと解釈された.この粒子は毛細血管壁の物質透過性の形態的研究に従来使われてきた標識物質と大きさはほぼ同じであるが, それ自身血液の生理学的構成要素である点で根本的にちがっていた.本研究で, 毛細血管内皮はいわゆる窓のほか2つ以上の小胞がつながってできる細管により貫かれ, 血管腔と組織腔とはこれを通して結ばれていることが明らかになった. 窓の隔膜と同様の構造が小胞の開口部および2つの小胞の癒合部にもみられた. これらの隔膜はコロイド粒子の通過をさまたげるもので, 生理学者のいう“small pore system” はこの隔膜に含まれると考えられた. 隔膜がすべてとり去られた場合, 細管は巨大な粒子をも通過させ得る. PALADEらのいう“transport in quanta”は本研究で明らかにされた内皮細胞を貫く細管系による物質の流出よりも効率が低いと想像され, 毛細血管内皮の物質透過が“transport in quanta”とちがうことを強調するためにこの論文では細管系による物質の流出は“transport in continuum”と呼ばれた.コロイド粒子は一般的には毛細血管の基底膜を通過するが腎糸球体ではこの膜は完全にこの粒子の通過を阻止していた.

著者

Junqueira L.C.U. Cossermelli W. Brentani R.

出版者

International Society of Histology and Cytology

雑誌

Archivum histologicum japonicum (ISSN:00040681)

巻号頁・発行日

vol.41, no.3, pp.267-274, 1978

被引用文献数

2 408

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Organs of fish, amphibian, reptile, bird and mammals when stained by Sirius Red and studied with polarization microscopy present different colors in regions where collagens I, II and III have been described.<br>Collagen type I presented a yellow, orange or red color while collagen type III appeared green. Collagen type II, present in cartilage and chondrosarcoma showed a variable color according to the tissue and the species. Its color and morphology however always permitted its clear distinction from collagens type I and type III.

著者

伊東 俊夫 柴崎 晋 高橋 嘉幸

出版者

International Society of Histology and Cytology

雑誌

Archivum histologicum japonicum (ISSN:00040681)

巻号頁・発行日

vol.24, no.3, pp.229-245, 1964

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In der Nebennierenrinde von eingm männlichen Nyctereutes procyonoides viverrinus wurde eine auffallend deutlich ausgeprägte Zona intermedia ('transitional zone', sudanophobe Zone) zufälligerweise gefunden und histologisch und cytologisch eingehend beobachtet. Im folgenden werden die wichtigsten Ergebnisse dieser Beobachtung zusammengefasst dargestellt. Bei der Fixierung wurde die Nebenniere als Ganzes parallel der Längsachse in dünne Stücke geschnitten, und danach in LEVIschem Osmiumgemisch und ZENKER-Formol fixiert. Die auf gewöhnliche Weise in Paraffin eingebetteten Gewebsstücke wurden in etwa 4μ dicke Serienschnitte zerlegt. Die Färbung geschah mit Azan, Eisenhämatoxylin nach HEIDENHAIN, KULL's Anilinfuchsin-Aurantia und Hämatoxylin (HANSEN)-Eosin. Einige Schnitte wurden mit Perjodsäure-SCHIFFscher Methode (PAS) behandelt, darauf mit Eisenhämatoxylin nachgefärbt.<br>1. Die Nebennierenrinde des Nyctereutes besteht, wie bei anderen Säugern, aus Zona glomerulosa, fasciculata und reticularis und zwischen der Glomerulosa und Fasciculata findet sich eine gut ausgeprägte Zona intermedia, die eine den ganzen Umfang der Rinde hindurch nahezu prallel der Oberfläche quer verlaufende dünne Schicht darstellt. Histologischer Bau und Anordnung der Rindenzellenstränge sind in der Glomerulosa und Fasciculata typisch. In den beiden Zonen enthalten die Rindenzellen im allgemeinen eine große Menge Lipoidgranula, die in den mit LEVIschem Gemisch fixierten Präparaten gewöhnlich geschwärzt erhalten sind, während sie aberauch öfters aufgelöst als kleine Vakuolen zum Vorschein kömmen können. Die Fasciculatazellen sind mit vielen Lipoidgranula am stärksten beladen und erscheinen im allgemeinen etws größer als Glomerulosazellen. Zwischen den ein Netz bildenden Zellensträngen der Zona reticularis finded man verhältnismäßig reichliche Bindegewebsfasern mit Bindegewebszellen. In dieser Zone sind die Rindenzellen klein, aber enthalten eben-falls Lipoidgranula. Die Häufigkeit des Vorkommens von dunklen degenerierten Rindenzellen ist je nach den Stellen der Rinde schwankend. Sie kommen in der Regel in der Zona reticularis am zahlreichsten vor, während sie aber in der Zona fasciculata, under Umständen sogar in der der Zona intermedia angrenzenden oberflächlichen Schicht in merklicher Zahl auftreten können. In der Zona glomerulosa findet man degenerierte Zellen kaum und in der Zona intermedia gar nicht.<br>2. Die Zona intermedia stellt eine klar ausgeprägte, den ganzen Umfang der Rinde hindurch fast kontinuierliche, dünne Schicht dar. In dieser Zone sind die Rindenzellen im allgemeinen arm an Cytoplasma und klein, die Kerne sind relativ groß, aber ihre Größe ist ziemlich schwankend; ihre Verteilung ist öfters unregelmäßig, sodaß miteinander dicht anliegende Kerne nicht selten auf das Vorhandensein von zwei-oder mehrkernigen Zellen hindeuten, Die Zellgrenze ist mit lichtmikroskopischer Beobachtung nicht immer festzustellen. Solche Rindenzellen sind im großen und ganzen ein- oder zweireihig angeordnet und belden dünne Zellstränge, welche meistenfalls parallel der Oberfläche der Rinde quer gestreckt sind. Die Zona intermedia besteht an den misten Stellen aus den von außen nach innen zwei- oder dreischichtig, selten einschichtig angeordneten Zellsträngen. Es ist ein bemerkenswertes Ergebnis, daß die Zona intermedia, wie bei der Zona reticularis, reich an Bindegewebe ist. So lassen sich einzelne Zellstränge von einer verhältnismäßig dicken Faserhaut umhüllen und zwischen den Zellsträngen finden sich locker verteilte Bindegewebsfasern mit relativ vielen Bindegewebszellen und Blutkapillaren

著者

Yuji Sonoda Kazunobu Sasaki

出版者

International Society of Histology and Cytology

雑誌

Archives of Histology and Cytology (ISSN:09149465)

巻号頁・発行日

vol.71, no.3, pp.155-161, 2008 (Released:2009-02-05)

参考文献数

15

被引用文献数

7 9

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This study used 100-μm thick paraffin sections stained by the ER-HR3 antibody to examine the three-dimensional surface morphology of the central macrophages of erythroblastic islets in the splenic red pulp of aged and pregnant mice. The ER-HR3-positive cells were the macrophages located at the center of the erythroblastic islets, and the number per unit of splenic area was almost constant until 30 days of age, thereafter showing a marked decrease. In pregnant females, the ER-HR3-positive macrophage number significantly increased and became approximately eight times higher than the control value. In aged virgin females, the islet macrophages were generally ovoid in cell profile, and shallow cup-shaped dents were formed on their cell surface. However, in pregnant females, the macrophages became larger in size, and cell socket structures, formed by long finger-like cytoplasmic processes, became prominent on their cell surface. The 3-D images, obtained from 100-μm thick paraffin sections, provided the clear morphological evidence of the activity of the islet macrophages in spleen erythropoiesis.

著者

粟屋 和彦 池田 輝正 田川 二秋

出版者

International Society of Histology and Cytology

雑誌

Archivum histologicum japonicum (ISSN:00040681)

巻号頁・発行日

vol.8, no.1, pp.81-86, 1955-03-20 (Released:2009-02-19)

参考文献数

10

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In an attempt to determine whether depolymerized desoxyribonucleic acid, a product of decomposed lymphocyte nuclei, may produce plasmacellular response in lymph nodes or not, the following experiments were carried out: Different amounts of desoxyribonucleic acid (DNA), i. e. 10mg and 2.5mg DNA dissolved in 1ml of physiological saline (pH was corrected by adding N/10-NaOH approximately to 7.0), were injected subcutaneously into the foot-pad of the right hind legs in a series of adult rabbits, and cellular response in the regional lymph nodes (popliteal nodes) was observed in sections. As control, 1ml of physiological saline was injected into the foot-pad of the left hind legs.Following DNA injection, a marked infiltration of pseudoeosinophilic leucocytes occurred in both the sites of injection and the regional lymph nodes within several hours, and then disappeared gradually after 48 hours. The plasmacytes which are normally present in the medullary cords of the nodes, also increased in number to some extent, coincident with the infiltration of pseudoeosinophiles. But their increase was not significant as compared with the controls, because injection of physiological saline alone produced a similar increase of plasmacytes (Tables 1 and 2). The cellular response in the sites of injection was almost the same as observed in the regional lymph nodes.It is concluded from these findings that depolymerized DNA, a product of decomposed lymphocyte nuclei, cannot be regarded as a primary factor to produce plasmacytic reaction in lymph nodes.

著者

鈴木 清之

出版者

International Society of Histology and Cytology

雑誌

Archivum histologicum japonicum (ISSN:00040681)

巻号頁・発行日

vol.32, no.4, pp.375-381, 1970

被引用文献数

4

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進行性筋ジストロフィー症患者 (32才男) の筋生検を行い, リングファイバーとその神経筋接合部を観察した. リングファイバーの筋線維は, 中心部は長軸方向に, また周辺部はそれをとりかこむ様に走り, 一部はみだれた配列をしめしていた. 筋小胞体は, 多少, 少ない様にみえた. トライアドは筋線維の横紋に関係なく不規則に分布していた. ミトコンドリアは, 筋鞘の近くに特に多くみられた.<br>リングファイバーの神経筋接合部は, 骨格筋のそれと似た像を呈するが, 結合ヒダは, もっと複雑な像を観察した.

著者

平 一男 武藤 博孝

出版者

International Society of Histology and Cytology

雑誌

Archivum histologicum japonicum (ISSN:00040681)

巻号頁・発行日

vol.44, no.4, pp.373-384, 1981 (Released:2009-02-20)

参考文献数

30

被引用文献数

8 4

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爬虫類に属するカナヘビ, ヤマカガシおよびイシガメの肝臓の伊東細胞を電子顕微鏡で観察した.観察したいずれの動物においても伊東細胞はディッセ腔内にあり, 通常脂肪滴を含んでいた. ブタの肝臓を飼料として飼育したイシガメの伊東細胞の脂肪滴は数も多く, 形も大きかった. 野外で捕獲したカナヘビとヤマカガシの伊東細胞の脂肪滴は数も少なく, 形の小さいものが多かった. ヤマカガシでは脂胞滴をもたない“空虚な伊東細胞”がしばしば観察された. 内腔の拡大した粗面小胞体がカナヘビとヤマカガシの伊東細胞にみられたが, イシガメにはみられなかった. 動物の栄養状態, とくに食餌中のビタミンAの含有量の多少が, このような相違をもたらしたものと思われる.