著者

赤田 良信 河野 貞子 山岸 正治 棚瀬 弥一郎

出版者

公益社団法人日本薬学会

雑誌

藥學雜誌 (ISSN:00316903)

巻号頁・発行日

vol.100, no.2, pp.212-215, 1980-02-25

被引用文献数

1

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A rapid method for determining paeonol in Moutan Cortex ("Mudanpi"-root bark of Paeonia moutan) was established by high-speed liquid chromatography. Paeonol was separated in a 25 cm column of Zorbax CN, using a Shimadzu-Du Pont Model LC-2 liquid chromatograph, with 50% acetonitrile as the desorption solution. Separation was completed within 7 min. Paeonol in Moutan Cortex was extracted with methanol and the extract was injected directly into the column. Paeonol content was estimated from previously prepared calibration curves based on standard paeonol. At 275 nm, the precision of the determination was about ±1% and the detection limit was about 2 ng (S/N ratio, 3). It was concluded that this method is useful for the evaluation of Moutan Cortex.

著者

崎谷 陽子 赤田 良信 河野 貞子 宮内 芳子

出版者

公益社団法人日本薬学会

雑誌

Chemical & pharmaceutical bulletin (ISSN:00092363)

巻号頁・発行日

vol.27, no.5, pp.1125-1129, 1979-05-25

被引用文献数

9

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A method was established by which glycyrrhizin and glycyrrhetinic acid present in plasma can be extracted with methanol and then separated and determined quantitatively within 10 min by means of high-speed liquid chromatography. Using this method, glycyrrhizin and glycyrrhetinic acid added to the plasma were recovered to satisfactory extents. An in situ recirculating perfusion technique showed that G is absorbed in rat small intestine in an apparent first-order process. There was no detectable amount of glycyrrhetinic acid in the blood after bolus injection of glycyrrhizin into the portal vein, althouth glycyrrhetinic acid was present in a detectable amount in the blood after oral administration. Since it is water-soluble and has a high molecular weight, glycyrrhizin is probably absorbed in the small intestine in the form of glycyrrhetinic acid. With the decline of glycyrrhetinic acid in the blood, there was a rise in the blood level of a substance which exhibited the same chromatographic behavior as glycyrrhizin. This substance appears to be a glucuronic acid conjugate formed as a metabolite of glycyrrhetinic acid, although it is not clear whether it is a mono-or diglucuronic acid conjugate or a mixture of the two. Glycyrrhizin injected into the portal vein was eliminated from the blood only slowly.