- 著者
- 立石 法史 中村 淳一 野中 裕司
- 出版者
- 公益社団法人 日本農芸化学会
- 雑誌
- 化学と生物 (ISSN:0453073X)
- 巻号頁・発行日
- vol.56, no.6, pp.408-413, 2018-05-20 (Released:2019-05-20)
- 参考文献数
- 26
動脈硬化性疾患は日本人の死因の25%近くを占め,その主たる危険因子である高血圧,糖尿病,脂質異常症などの生活習慣病の発症には,肥満,特に内臓脂肪蓄積が深く関与していると指摘されている.これらの予防・改善には,適度な運動とバランスの良い食生活が大切であるが,現代社会の多忙な日常生活においては,適切かつ継続的な実践は必ずしも容易ではないため,保健機能食品の効果的な活用は国民の健康の維持・増進への一助となることが期待される.そこでわれわれは,肥満や内臓脂肪蓄積の予防・改善に役立ち,無理なく飲み続けられる日頃慣れ親しんだ茶飲料での特定保健用食品を開発することを目指した.
- 著者
- 福井 祐子 岩下 孝 浅見 純生 野中 裕司 前田 満 橋本 文雄 木曽 良信
- 出版者
- 天然有機化合物討論会
- 雑誌
- 天然有機化合物討論会講演要旨集
- 巻号頁・発行日
- no.50, pp.475-480, 2008-09-01
Oolong tea is a semi-fermented tea, which has been manufactured from leaves of Camellia sinensis (L) O. Kuntze, same as non-fermented Japanese or Chinese green teas. Major components of fresh tea leaves are caffeine and catechins such as epigallocatechin-3-O-gallete (EGCG). During the fermentation process, polyphenol oxidase (PPO) in tea leaves oxidizes the catechins into theasinensin and oolongtheanin. In addition, oolonghomobisflavans are known as condensation products of catechins by heat treatment. The aim of this study is to quantify these minor polymerized polyphenols by LC-MS/MS, and address their biological activities. Quantification of polyphenols by LC-MS/MS Theasinensin (TSN), oolongtheanin gallate (OTNG), and oolonghomobisflavan (OHBF) were synthesized from EGCG. Two new trimers and tetramers of oolonghomobisflavan were synthesized along the previous synthesis method of OHBF, and then their structures were determined by MS and NMR spectroscopy. Quantification of these compounds was performed by LC-MS/MS in the mode of multiple reaction monitoring (MRM). Compounds other than TSN-A were of small quantities in oolong tea leaves. Quantification of polymerized polyphenols was not performed with HPLC UV monitoring, but by LC-MS/MS method. Nine kinds of oolong teas in different fermentation levels were extracted and analyzed. The very small amount of trimers and tetramers of OHBF were included in "Black oolong tea", therefore, it was necessary to make concentrated solutions of these by column separation, and quantified the real amount by analyzing them. Biological activities of polymerized polyphenols Pancreatic lipase inhibitory activity of oolong tea polymerized polyphenols (OTPP) and some catechin dimmers have been already reported. In this study, new trimers and tetramers also exhibited strong inhibitory activity. Besides OTPP, the polymerized polyphenols, TSN, OTNG, and OHBF also have α-glucosidase inhibitory activity. These results suggest that oolong tea prevents the elevations of triglyceride in blood from meal fat, as well as inhibit the absorption of sugars derived from carbohydrates.