1 0 0 0 OA 若年健常女性におけるL-カルニチンによる空腹感軽減効果の検討
- 著者
- 永井 成美 亀田 菜央子 小橋 理代 西田 美奈子 堀川 千賀 江川 香 吉村 麻紀子 北川 義徳 阿部 圭一 木曽 良信 坂根 直樹 小谷 和彦 森谷 敏夫
- 出版者
- 公益社団法人 日本栄養・食糧学会
- 雑誌
- 日本栄養・食糧学会誌 (ISSN:02873516)
- 巻号頁・発行日
- vol.60, no.5, pp.257-264, 2007-10-10 (Released:2009-01-30)
- 参考文献数
- 22
L-カルニチンがヒトの空腹感に及ぼす影響を明らかにするために, 若年健常女性12名 (21.3±0.3歳) を対象として, L-カルニチン300mgを含有するフォーミュラ食と通常のフォーミュラ食を用いた二重盲検プラセボ対照試験による検証を行った (ウォッシュアウト : 1週間)。前夜からの絶食の後, フォーミュラ食を朝食として摂取させ, 食前および食後6時間まで満腹感スコア (ビジュアルアナログスケールズ ; VASs), 唾液コルチゾール, 血清カルニチン濃度, 血糖値, および心拍変動パワースペクトル解析を用いた自律神経活動指標を経時的に測定した。実験結果から, L-カルニチン摂取により主観的空腹感が軽減される可能性があること, および, 空腹感の軽減には血清総カルニチン濃度が関連していることが示唆された。さらに, 唾液コルチゾールはL-カルニチン摂取30分後, 2時間後には低値を示したが, 空腹感軽減との明確な関連は明らかではなかった。
- 著者
- 福井 祐子 岩下 孝 浅見 純生 野中 裕司 前田 満 橋本 文雄 木曽 良信
- 出版者
- 天然有機化合物討論会
- 雑誌
- 天然有機化合物討論会講演要旨集
- 巻号頁・発行日
- no.50, pp.475-480, 2008-09-01
Oolong tea is a semi-fermented tea, which has been manufactured from leaves of Camellia sinensis (L) O. Kuntze, same as non-fermented Japanese or Chinese green teas. Major components of fresh tea leaves are caffeine and catechins such as epigallocatechin-3-O-gallete (EGCG). During the fermentation process, polyphenol oxidase (PPO) in tea leaves oxidizes the catechins into theasinensin and oolongtheanin. In addition, oolonghomobisflavans are known as condensation products of catechins by heat treatment. The aim of this study is to quantify these minor polymerized polyphenols by LC-MS/MS, and address their biological activities. Quantification of polyphenols by LC-MS/MS Theasinensin (TSN), oolongtheanin gallate (OTNG), and oolonghomobisflavan (OHBF) were synthesized from EGCG. Two new trimers and tetramers of oolonghomobisflavan were synthesized along the previous synthesis method of OHBF, and then their structures were determined by MS and NMR spectroscopy. Quantification of these compounds was performed by LC-MS/MS in the mode of multiple reaction monitoring (MRM). Compounds other than TSN-A were of small quantities in oolong tea leaves. Quantification of polymerized polyphenols was not performed with HPLC UV monitoring, but by LC-MS/MS method. Nine kinds of oolong teas in different fermentation levels were extracted and analyzed. The very small amount of trimers and tetramers of OHBF were included in "Black oolong tea", therefore, it was necessary to make concentrated solutions of these by column separation, and quantified the real amount by analyzing them. Biological activities of polymerized polyphenols Pancreatic lipase inhibitory activity of oolong tea polymerized polyphenols (OTPP) and some catechin dimmers have been already reported. In this study, new trimers and tetramers also exhibited strong inhibitory activity. Besides OTPP, the polymerized polyphenols, TSN, OTNG, and OHBF also have α-glucosidase inhibitory activity. These results suggest that oolong tea prevents the elevations of triglyceride in blood from meal fat, as well as inhibit the absorption of sugars derived from carbohydrates.