著者

馬場 清喜 村川 武志

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.64, no.4, pp.285-289, 2022-12-15 (Released:2022-12-17)

参考文献数

16

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It is difficult to perform the thermodynamic analysis of conformational changes occurring in protein crystals by conventional cryogenic X-ray crystallography because flash-cooled crystals have ambiguous information about temperature. We have developed a humid air and glue-coating(HAG)method in which the crystals are coated with a water-soluble polymer to control humidity. This method realized X-ray crystallographic analysis with controlled temperature and humidity in a non-cryogenic environment. In this paper, we report details of the HAG method and the thermodynamic analysis of conformational changes of copper-containing amine oxidase using the HAG method.