- 日本結晶学会誌 (ISSN:03694585)
- vol.63, no.4, pp.267-272, 2021-12-15 (Released:2021-12-18)
In March 2018, KEK obtained a 200 kV cryogenic electron microscopy(cryo-EM; a Talos Arctica G2 with a Falcon 3EC direct electron detector)and prepared a pipeline for solving high-resolution structures of macromolecular biomolecules, such as proteins, DNA, and RNA. In October 2018, the cryo-EM facility in KEK opened to academic and industrial users. Our mission is three-fold:to provide cryo-EM machine time for external users, to support grid-prep and data collection(and single particle analysis, if needed), and to help users acquire cryo-EM skills. One remarkable result is the single-particle analysis of an 860-kDa enzyme at 2.05 angstrom resolution, which suggested that we had established a proper protocol for cryo-EM operation and single-particle analysis. We will continue to support external users and carry out cryo-EM single particle analysis of macromolecular biomolecules.