著者

代田 稔 麻生 健治 岩淵 明

出版者

日本細菌学会

雑誌

日本細菌学雑誌 (ISSN:00214930)

巻号頁・発行日

vol.17, no.12, pp.917-927, 1962

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It was revealed that GYB (glucose yeast broth) agar devoid of calcium carbonate was best fitted for counting the total number of viable bacteria in feces, regardless of their kinds. Widely used TGC (thioglycollate) broth was found far inferior to GYB-agar as regards fo: accuracy and sensitivity.<BR>Quantitative estimation and isolation of lactobacilli from human feces were performed most excellently when LBS (Lactobacillus selective medium) 1 agar was used under anaerobic condition. IBS 1 agar is a modified medium of Rogosa's medium (1951), developed by the authors in order for better isolation of lactobacilli. It contains, besides LBS medium, liver extract, lactose and cysteine.<BR>As for estimation of lactic acid bacteria (lactobacilli and streptococci), our tomato juice agar containing 0.1% furan-acrylic acid gave most excellent results.<BR>Concomitant studies on various selective media currently used for other microbes confirmed that for enteric bacteria, MacConkey, Desoxycholate agar, SF (Streptococcus faecalis) medium as well as Staphylococcus medium No.110; for yaests and Candida, potato glucose agar or CGS (Sabouraud's medium containing guanoflacine) agar; were excellent selective media, respectively.

著者

代田 稔 麻生 健治 岩淵 明

出版者

日本細菌学会

雑誌

日本細菌学雑誌 (ISSN:00214930)

巻号頁・発行日

vol.21, no.5, pp.274-283, 1966

被引用文献数

1 14

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50ml of fermented milk, containing 1 to 2&times;10⁸/ml of live Lactobacillus acidophilus strain Shirota (LAS) and 180ml of market milk were orally administration daily to healthy infants (2-6 years) for 35 days, and its effect on the constitution of microflora in feces was investigated, using appropriate selective media.<br>In control group, normal microflora in one gram of feces was found to consitute of 10⁸-10¹⁰ of Bifidobacterium classified to three physiological type, 10⁶-10⁹ of enterobacteria group in which E. coli was predominant, 10³-10⁵ of lactobacillus group consisting of 60% of L. acidophilus and other lactobacilli (L. plantarum, L. casei and heterofermentives), 10⁴-10⁷ of enterococci, and 10-10⁴ yeasts.<br>During the first 7 days of adminstration, lactobacillus in feces increased to 10⁶-10⁸/gram and this level persisted during the whole administration period. This lactobacillus was serologically identified to be LAS. In contrast, enterobacterial group and enterococci decreased by a facter of 1/100 and 1/50, respectively. When administration was stopped, LAS decreased gradually and one to two weeks after became undetectable, while the suppressed groups recovered to original level. Concomitant with this change, the pH of the feces rised from 5.0-7.5 to original value, 6.7-8.7. Other members in feces were not siginificantly affected through the period of experiment.<br>These results led to the conclusion that LAS survives in human intestinal tract though it does not establish permanent residence, and alter the consititution of microflora, being especically antagonistic against E. coli and enterococci. The antagonistic activity of LAS is discussed.