著者

Ahmad M. Al Athamneh. Supatta Chawalitpong 鈴木 湧太 山口 大樹 中村 宗一郎 片山 茂

出版者

Japanese Society of Food Chemistry

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.26, no.2, pp.91-98, 2019 (Released:2019-08-28)

参考文献数

24

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Fag e 1 is a 22 kDa globulin found in common buckwheat (Fagopyrum esculentum) and it is known to be one of the major allergens causing severe allergic symptoms. In this study, we successfully obtained recombinant Fag e 1 using the Pichia expression system and prepared an allergen-specific hypoallergenic agent by the controlled dry-heating phosphorylation of Fag e 1 (P-Fag e 1). Then, we investigated if P-Fag e 1 can be useful as an immunomodulator in Fag e 1-sensitized mice. For this, P-Fag e 1 was orally administrated into Fag e 1-sensitized mice for 6 weeks, and then these mice were challenged with Fag e 1. We observed a significant reduction in the histamine release in addition to diminished production of total as well as specific IgE in the P-Fag e 1-treated mice. In contrast, total IgA level increased by the treatment with P-Fag e1. The levels of the IL-4 cytokines from both spleen and Peyer’s patches were significantly decreased in P-Fag e 1 treated mice. Additionally, the population of T follicular helper cells (Tfh cells) was increased in the P-Fag e 1 treated group. The suppression of IgE production in the Fag e1 treated group might be due to the enrichment of the Tfh cells and IgA production. Therefore, it could be proposed that P-Fag e 1 is an allergen-specific immunomodulator in mice allergic to Fag e 1.