- 著者
- Yuki Hara Kenta Adachi Shunsuke Kagohashi Kazuo Yamagata Hideyuki Tanabe Shinji Kikuchi Sei-Ichi Okumura Akatsuki Kimura
- 出版者
- THE SOCIETY OF CHROMOSOME RESEARCH
- 雑誌
- Chromosome Science (ISSN:13441051)
- 巻号頁・発行日
- vol.19, no.1-4, pp.43-49, 2016 (Released:2017-06-26)
- 参考文献数
- 22
Across species, eukaryotic chromosomes share common features at the molecular level. However, common features at the cellular level are not well investigated. A correlation has been suggested between the linear packing ratio of mitotic chromosomes and the intra-nuclear DNA density, by comparing these values in the nematode Caenorhabditis elegans. In this study, these values were measured and compared among several metazoan and plant species. The obtained values corroborated the correlation proposed in the previous study, supporting the theory that intra-nuclear DNA density is a common regulator of chromosome condensation. Moreover, the comparison among different species suggested a correlation between the length of a mitotic chromosome and the nuclear volume to the power of 2/3. Given this observation, we speculate that: (i) a rate-limiting component defines the length of a mitotic chromosome that is proportional to the nuclear surface area, and (ii) such regulation of the mitotic chromosomal length may play a role in maintaining the ratio between the cell size and the metaphase plate.
- 著者
- Manuel Alejandro CAMPOS MEDINA Kenji IEMURA Akatsuki KIMURA Kozo TANAKA
- 出版者
- Biomedical Research Press
- 雑誌
- Biomedical Research (ISSN:03886107)
- 巻号頁・発行日
- vol.42, no.5, pp.203-219, 2021-09-21 (Released:2021-09-21)
- 参考文献数
- 49
- 被引用文献数
- 1
Chromosome oscillation during metaphase is attenuated in cancer cell lines, concomitant with the reduction of Aurora A activity on kinetochores, which results in reduced mitotic fidelity. To verify the correlation between Aurora A activity, chromosome oscillation, and error correction efficiency, we developed a mathematical model of kinetochore-microtubule dynamics, based on stochastic attachment/detachment events regulated by Aurora A activity gradient centered at spindle poles. The model accurately reproduced the oscillatory movements of chromosomes, which were suppressed not only when Aurora A activity was inhibited, but also when it was upregulated, mimicking the situation in cancer cells. Our simulation also predicted efficient correction of erroneous attachments through chromosome oscillation, which was hampered by both inhibition and upregulation of Aurora A activity. Our model provides a framework to understand the physiological role of chromosome oscillation in the correction of erroneous attachments that is intrinsically related to Aurora A activity.