- 著者
-
Takeshi YOSHINARI
Thillaiampalam SIVAKUMAR
Masahito ASADA
Badgar BATTSETSEG
Xiaohong HUANG
Dinh Thi Bich LAN
Tawin INPANKAEW
Adrian P. YBAÑEZ
Andy ALHASSAN
Oriel M. M. THEKISOE
Alan Caine Costa DE MACEDO
Hisashi INOKUMA
Ikuo IGARASHI
Naoaki YOKOYAMA
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.75, no.2, pp.211-214, 2013 (Released:2013-03-01)
- 参考文献数
- 16
- 被引用文献数
-
6
21
Babesia ovata is a tick-transmitted hemoprotozoan parasite that infects cattle. In our study, bovine blood samples (n=2,034) were collected from 10 different countries (Brazil, China, Ghana, Japan, Mongolia, the Philippines, South Africa, Sri Lanka, Thailand and Vietnam) and DNA extracted. The DNA samples were screened using an established and specific polymerase chain reaction (PCR) assay targeting the Apical membrane antigen 1 (AMA-1) gene. Parasite DNA was detected among samples collected from Japan, Mongolia and Thailand. Sequence analyses confirmed that the PCR assay detected only B. ovata AMA-1, and that amplicons from different geographical locations were conserved. Our findings highlight the importance of designing adequate strategies to control B. ovata infection in Japan, Mongolia, and Thailand.