- 著者
- Xuelong Jin Bin Liang Feifei Wang Zhiwen Zhang
- 出版者
- Japan Brain Science society
- 雑誌
- 脳科学誌 (ISSN:13415301)
- 巻号頁・発行日
- vol.39, pp.32-40, 2012-10-30 (Released:2017-06-01)
- 参考文献数
- 22
Objective Those who suffer from brainstem hemorrhage complicated by stress ulcer hemorrhage have a high mortality. This research inquire the ideal surgical method to observe microcirculation of duodenal mucosal villi and the dynamic changes in the microcirculation of rabbit's duodenal mucosal villi under brainstem hemorrhage conditions with a red color coordinate, aiming at establishing a mechanism for testing the microcirculation of the digestive tract and providing a reference index for the prevention and treatment of brainstem hemorrhage complicated by gastrointestinal bleeding. Method In this experiment, thirty male Japanese white rabbits with long ears (1.5kg to 1.8kg) were anaesthetized by 25% Urethane in vein, and simple disinfected in the condition of animal laboratory. They were given a cranial fenestration operation for an acute brainstem hemorrhage animal model and an enterstomy on the abdominal wall was made. Microcirculation microscopic blood vessel telecamera and microcirculation TV video system were used to continuously detect the congestion of the duodenal mucosal villi under brainstem hemorrhage conditions. Computer and photoshop software were used to continuously analyze the changes with time of the red color coordinate of the same villus on the same point. Result The red color (pallor) of duodenal mucosa reduced, along with minimal morphological changes of duodenal mucosa after brainstem hemorrhage. Conclusions Under the condition of this experiment, this surgical method can establish the experimental animal model to observe microcirculation of duodenal mucosal villi, and reduction of the red color coordinate with time verified the pallor of duodenal mucosa after brainstem hemorrhage in a rabbit model and reflected the sharp changes of mucosa's blood stream which is the most important preventive factor to stress ulcer under acute brainstem hemorrhage conditions. And these would become the pathophysiological basis of upper digestive tract ulcer.
- 著者
- Xuelong Jin Bin Liang
- 出版者
- Japan Brain Science society
- 雑誌
- 脳科学誌 (ISSN:13415301)
- 巻号頁・発行日
- vol.38, pp.35-44, 2012-03-30 (Released:2017-06-01)
- 参考文献数
- 16
Objective: Severe cerebral contusion will cause a functional defect in partial central nerve and will lower patients' living quality. The above problem is still a difficult problem unsolved in clinical treatment. This study is going to clarify the methodology of rabbit's brain tissue transplantation, including the way of operation and the law of microcirculatory formation. Method: Twenty healthy male, Japanese white rabbits with long ears (about three months after birth) of clean degree, weighting between 1.2kg and 1.3kg, were anaesthetized by 3% pentobarbital sodium in vein before receiving an intracerebral transplantation operation. A window was opened on their parietal bone symmetrically, and the cortical brain tissues on the symmetrical areas (on the left and right side) of the rabbits' parietal cortex areas were exchanged and transplanted. Gentamycin sulfate was injected each day to resist infection. Ten and twenty days later, an observation was made as to the survival of the transplanted area and host brain tissue. A microcirculation color camera system was used to analyze the pictures of angiogenesis. With regard to the survival of transplanted brain tissues, their changes in micromorphology were observed. Besides, pathological sections were also prepared to determine their surviving conditions on cell level. Results: (1) Surgical operation has contributed to a satisfactory morphological anastomosis between transplanted brain tissues and host brain tissues. (2) Micro-blood vessel loops were observed to have formed on the section (host side) of rabbits' parietal cortex areas, which had been excised partially when transplantation was not filled. Marker was implanted into the transplanted brain tissues to confirm the possibility of the regeneration of a microcirculation among brain tissues, which had been exsomatized completely. (3) Analysis of the pathological sections of the transplanted brain tissues showed traces of surviving nervous cells. Conclusions: Under given conditions, nervous cells' survival can be maintained by transplanted brain tissues and can be nourished by angiogenesis and characteristic microcirculation connections with host brain tissues.