- 著者
- Kenji NARUSE Yan-Shi QUAN Su-Min CHOI Chang-Sik PARK Dong-Il JIN
- 出版者
- The Society for Reproduction and Development
- 雑誌
- Journal of Reproduction and Development (ISSN:09168818)
- 巻号頁・発行日
- vol.53, no.3, pp.679-684, 2007 (Released:2007-07-07)
- 参考文献数
- 19
- 被引用文献数
- 8 8
This study was carried out to investigate the effects of minimum essential medium (MEM) vitamins during in vitro maturation (IVM)/in vitro culture (IVC) of porcine nuclear transfer (NT) embryos on subsequent developmental capacity in vitro. Porcine cumulus-oocyte complexes (COCs) were divided into five groups, matured for 44 h in maturation medium with various concentrations of MEM vitamins (0, 0.05, 0.1, 0.2 and 0.4%), and observed for maturation rate. Also, COCs were matured in NUSU-23 media without MEM vitamins for 44 h and cultured in PZM-3 media with various concentrations of MEM vitamins (0, 0.05, 0.4 and 1.0%) for 6 days following nuclear transfer. Factorial (IVM/IVC) experiments were also performed in NCSU-23 medium with or without 0.05% MEM vitamins and PZM-3 medium with or without 0.4% MEM vitamins. They were then tested by examining in vitro development of the porcine reconstructed embryos. The maturation rates of the COCs treated with the MEM vitamins did not differ significantly among the MEM vitamin-treated groups. Addition of vitamins to culture medium did not affect development of porcine reconstructed embryos in vitro. However, addition of low concentrations of MEM vitamins only to maturation medium increased (P<0.05) the proportion of NT embryos developing into blastocysts compared with the control group. Addition of MEM vitamins to IVC medium did not enhance the developmental rate compared with the control group. Thus, addition of MEM vitamins to IVM medium could improve subsequent blastocyst development of porcine NT embryos.
- 著者
- Chang-Sik Park
- 出版者
- The Society of Physical Therapy Science
- 雑誌
- Journal of Physical Therapy Science (ISSN:09155287)
- 巻号頁・発行日
- vol.30, no.6, pp.835-839, 2018 (Released:2018-06-12)
- 参考文献数
- 20
- 被引用文献数
- 12
[Purpose] To establish the test-retest reliabilities, minimal detectable change of the Short form Barthel Index and associations with stroke-specific impairments. [Subjects and Methods] The Short form-Barthel Index assessment was tested on 24 chronic stroke patients twice, 7 days apart. A relative reliability index (ICC2,1), Weighted Kappa Coefficients was used to examine the level of agreement of test-retest reliability for SF-BI, Absolute reliability indices, including the standard error of measurement and the minimal detectable change. The validity was demonstrated by spearman correlation of SF BI-total score with Postural Assessment Scale for Storke, Fugl Meyer Assessment. [Results] There was excellent agreement between test-retest for individual items of BI and total score ICC2,1=0.91 and it all showed acceptable SEM and MDC were 2.83 score, 7.84 score respectively. The item-to-total correlations were all significant, ranging from r=0.83–0.92. SF-BI showed good internal consistency. Individual items also possessed high internal consistency 0.82–0.86. The SF-BI and total score were demonstrated high concurrent validity with the PASS, FMA. [Conclusion] This study has demonstrated that the SF-BI is a useful instrument with high test-retest reliability, Absolute reliability indices, internal consistency and validity.