著者

Aya Iwamoto Tomoyo Nakamura Naoki Narisawa Yukimasa Kawasaki Shin Abe Yasuyoshi Torii Hidenobu Senpuku Fumio Takenaga

出版者

Japanese Society for Food Science and Technology

雑誌

Food Science and Technology Research (ISSN:13446606)

巻号頁・発行日

vol.24, no.1, pp.129-137, 2018 (Released:2018-03-24)

参考文献数

33

被引用文献数

3 5

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Our previous study indicated that certain commercially available proteases inhibit biofilm formation by cariogenic streptococci. Natto made from soybeans cultured with Bacillus subtilis natto contains an abundance of proteolytic enzymes. In this study, we investigated the correlation between the protease activity of extracts from 36 commercially available natto products and the inhibition of biofilm formation. The biofilm inhibitory effect was found to correlate with the level of protease activity in the natto extracts, without reducing the viable cell numbers. The natto extract markedly inhibited the production of a water-insoluble glucan by Streptococcus mutans, which is the main agent involved in the formation of biofilm by cariogenic streptococci. The characteristics of the protease present in the extract were similar to those of nattokinase. Our results indicate that the protease activity exhibited by extracts of the Japanese fermented food natto reduces the risk of caries by inhibiting biofilm formation.

著者

Manami Kimijima Kengo Mandokoro Yuki Ichikawa Mizuki Tokumaru Naoki Narisawa Fumio Takenaga

出版者

Japanese Society for Food Science and Technology

雑誌

Food Science and Technology Research (ISSN:13446606)

巻号頁・発行日

vol.28, no.3, pp.267-273, 2022 (Released:2022-05-20)

参考文献数

26

被引用文献数

2

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Natto is a fermented food made from soybeans using Bacillus subtilis natto. B. subtilis is present in honey. In this study, we evaluated the genetic and enzymatic characteristics of B. subtilis obtained from commercial honey and its suitability for natto fermentation. Three bacterial strains isolated from honey samples of different origins were found to be highly homologous to B. subtilis via 16S rDNA analysis. Similar to B. subtilis natto, all three isolates were biotin auxotrophs and possessed two insertion sequences. Genotyping by random amplified polymorphic DNA-polymerase chain reaction and enzyme activity analysis showed that the isolates were genetically different from the commonly used natto strains Miyagino, Takahashi, and Naruse. The three isolates are highly suitable for natto fermentation. These results indicate that honey is a source of B. subtilis natto that is potentially useful in the food industry.