著者

Emdadul Haque Eiji Yamamoto Kenta Shirasawa Hiroaki Tabuchi Ung-Han Yoon Sachiko Isobe Masaru Tanaka

出版者

Japanese Society for Root Research

雑誌

Plant Root (ISSN:18816754)

巻号頁・発行日

vol.14, pp.11-21, 2020 (Released:2020-10-09)

参考文献数

31

被引用文献数

6

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Genetic studies on the purple-fleshed sweetpotato (Ipomoea batatas L.), which is rich in anthocyanin (AN) in the storage root, were performed by polyploid GWAS based on the allele dosage probability using 59,675 SNPs obtained from 94 F1 progenies between the cultivars 'Konaishin' (which has a high yield but no AN) and 'Akemurasaki' (which has a high AN content but low to moderate yield). The distribution of relative AN content was highly biased, with 60% of clones showing a low to undetectable level (A530 < 0.5). Fifty-nine SNPs from six signals on homologous groups (HGs) 3, 5 (one major and one smaller signal), 7, 13, and 15 were strongly associated with the relative AN content. Twelve SNPs from the major signal and one from the smaller signal of HG 5 were further detected by QTL analysis. In a database search of the AN biosynthesis gene, transcription factors IbMYB1 and IbWD40 and AN structural genes IbF3H and IbDFR were located on HG 5, suggesting that an SNP marker or markers from HG 5 might be tightly linked to candidate gene(s) homologous to one of these transcription factors and AN structural genes as a major factor in determining AN accumulation in the storage roots. These results would enhance our understanding of the underlying genetic basis of AN accumulation in the storage roots of sweetpotatoes, and the SNP markers found here, especially 13 SNPs from HG 5, would be a potential platform for future marker-assisted selection for breeding high-AN sweetpotato varieties.

著者

Emdadul Haque Hiroaki Tabuchi Yuki Monden Keisuke Suematsu Kenta Shirasawa Sachiko Isobe Masaru Tanaka

出版者

Japanese Society of Breeding

雑誌

Breeding Science (ISSN:13447610)

巻号頁・発行日

pp.19099, (Released:2020-05-19)

被引用文献数

7

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While sweetpotato (Ipomoea batatas L.) improvement has generally been done by field-based selection, molecular genetic studies on traits of interest, i.e., molecular markers are needed for enhancing the breeding program of this world’s 7th most important crop, as such markers facilitate marker-assisted selection. Here, we performed a combined approach of QTLs analyses and GWAS of storage root β-carotene content (BC), dry-matter (DM) and starch content (SC) using the genetic linkage maps constructed with 5,952 and 5,640 SNPs obtained from F1 progenies between cultivars ‘J-Red’ and ‘Choshu’. BC was negatively correlated with DM (r = –0.45) and SC (r = –0.51), while DM was positively correlated with SC (r = 0.94). In both parental maps, a total of five, two and five QTL regions on linkage groups 7 and 8 were associated with BC, DM and SC, respectively. In GWAS of BC, one strong signal (P = 1.04 × 10–9) was observed on linkage group 8, which co-located with one of the above QTL regions. The SNPs markers found here, particularly for β-carotene, would be useful base resources for future marker-assisted selection program with this trait.