2 0 0 0 OA Molecular cloning of canine Wilms’ Tumor 1 for immunohistochemical analysis in canine tissues
- 著者
- Osamu SAKAI Masashi SAKURAI Hiroki SAKAI Masahito KUBO Hiroko HIRAOKA Kenji BABA Masaru OKUDA Takuya MIZUNO
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.17-0229, (Released:2017-06-12)
- 被引用文献数
- 5
Wilms’ tumor 1 (WT1) expression has been investigated in various human cancers as a target molecule for cancer immunotherapy. However, few studies have focused on WT1 expression in dogs. Firstly, cDNA of canine WT1 (cWT1) was molecularly cloned from normal canine kidney. The cross-reactivity of the anti-human WT1 monoclonal antibody (6F-H2) with cWT1 was confirmed via Western blotting using cells overexpressing cWT1. Immunohistochemical staining revealed that cWT1 expression was detected in all canine lymphoma tissues and in some normal canine tissues, including the kidney and lymph node. cWT1 is a potential immunotherapy target against canine cancers.
- 著者
- Masaya IGASE Takako SHIMOKAWA MIYAMA Satoshi KAMBAYASHI Yumiko SHIMOYAMA Hiroko HIRAOKA Yumi HIRATA Miki IWATA Kenji BABA Takuya MIZUNO Masaru OKUDA
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.15-0156, (Released:2015-12-04)
- 被引用文献数
- 7
A 10-year-old female spayed mixed breed cat with a subcutaneous mass on the right hind limb-was revealed with bimodal monoclonal gammopathy composed of IgA by immunoelectrophoresis and immunofixation. Approximately 1 month after referral, the cat died due to renal failure. Postmortem immunohistopathologic evaluation of the subcutaneous mass revealed neoplastic cell proliferation of plasma cells and giant myeloma cells. Neoplastic cells were also present in the liver and spleen. These results led to the diagnosis of a rare case of feline myeloma-related disorders with extramedullary plasmacytoma infiltrating in multiple locations. This report emphasizes the necessity to accumulate cases with similar clinicopathologic findings in the future.
1 0 0 0 OA Establishment of Five Canine Lymphoma Cell Lines and Tumor Formation in a Xenotransplantation Model
- 著者
- Saori UMEKI Yasuo EMA Ryoichi SUZUKI Masahito KUBO Toshiharu HAYASHI Yasuhiko OKAMURA Jumpei YAMAZAKI Hajime TSUJIMOTO Kenji TANI Hiroko HIRAOKA Masaru OKUDA Takuya MIZUNO
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.75, no.4, pp.467-474, 2013 (Released:2013-05-02)
- 参考文献数
- 11
- 被引用文献数
- 5 26
Five novel, canine lymphoma cell lines (Ema, CLC, CLK, Nody-1 and UL-1) were established from dogs suffering from lymphoma and characterized in vitro and in vivo. All cell lines, except CLC, were characterized with T-cell phenotypes, by flow cytometric analysis and polymerase chain reaction for antigen receptor rearrangement. Cell proliferation rates and transcriptional levels of MYC, PTEN, KIT and FLT3 varied between each cell line. Intraperitoneal xenotransplantation of Ema, CLC, Nody-1 and UL-1 lymphoma cell lines into NOD/SCID mice induced ascites, intraperitoneal tumors and severe infiltration of lymphoma cells into the pancreas and mesentery. Establishment of novel canine lymphoma cell lines with different characteristics is critical for elucidating the pathophysiology of canine lymphoma and improving current therapies.
- 著者
- Takumi OKAWA Hiroko HIRAOKA Yuko WADA Kenji BABA Kazuhito ITAMOTO Takuya MIZUNO Masaru OKUDA
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.1112200735, (Released:2011-12-27)
- 被引用文献数
- 4 5
The second malignancies are frequent complications in the human patient with chronic lymphocytic leukemia (CLL). However, the clinical details and outcome of this phenomenon were unclear in the canine counterparts. Here we report a dog with high-grade lymphoma concurrent with T-cell CLL. A 10-year-old male golden retriever presented with lymphadenopathies. The lymph nodes contained large-sized lymphocytes, raising suspicion of high-grade lymphoma. Meanwhile, small-lymphocytic lymphocytosis in the peripheral blood was consistent with CLL. Interestingly, molecular biological analyses revealed that CLL cells were T-cell type, whereas lymphoma cells were B-cell type. Chemotherapy using the L-VCA short protocol was effective for 155 days, but the dog died on day 194 after diagnosis, despite rescue therapies.